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HSP22 antibody

Anti-HSP22 antibody used in Western Blot (WB). GTX64490
Anti-HSP22 antibody used in IHC (Paraffin sections) (IHC-P). GTX64490
Anti-HSP22 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64490

Cat. No. GTX64490

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human HSPB8 (NP_055180.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family B (small) member 8 , CMT2L , DHMN2 , E2IG1 , H11 , HMN2 , HMN2A , HSP22

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-HSP22 antibody used in Western Blot (WB). GTX64490

GTX64490 WB Image

WB analysis of various sample lysates using GTX64490 HSP22 antibody.
Dilution : 1:3000
Loading : 25μg per lane

Anti-HSP22 antibody used in IHC (Paraffin sections) (IHC-P). GTX64490

GTX64490 IHC-P Image

IHC-P analysis of rat heart tissue using GTX64490 HSP22 antibody.
Dilution : 1:100

Anti-HSP22 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64490

GTX64490 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX64490 HSP22 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399