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Hsp90 beta antibody

Anti-Hsp90 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79247
Anti-Hsp90 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79247
Anti-Hsp90 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX79247
Anti-Hsp90 beta antibody used in Western Blot (WB). GTX79247
Anti-Hsp90 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79247
Anti-Hsp90 beta antibody used in Flow cytometry (FACS). GTX79247
Anti-Hsp90 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX79247
Anti-Hsp90 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX79247
Anti-Hsp90 beta antibody used in Western Blot (WB). GTX79247
Anti-Hsp90 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79247
Anti-Hsp90 beta antibody used in Immunoprecipitation (IP). GTX79247

Cat. No. GTX79247

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IHC

Reactivity

 Human, Mouse, Rat, Primate
Package
100 μg ($399)
See all Hsp90 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:20000
ICC/IF 10-20 μg/ml
IHC-P Assay dependent
IHC-Fr 10 μg/ml
FACS 1-20 μg/ml
IP 2 μg
IHC Assay dependent
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Product Note

This antibody does not detect HSP86. The antibody is capable of precipitating HSP84 that is complexed with other proteins such as the aryl hydrocarbon (Ah) receptor.

PROPERTIES

Form

Liquid

Buffer

buffer, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to residues P(2) E E V H H G E E E V E(13) of mouse HSP84.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_11166571

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein 90 alpha (cytosolic), class B member 1 , 90kDa , AL022974 , C81438 , Hsp84 , Hsp84-1 , Hsp90 , Hspcb

Cellular Localization

Cytoplasm,Nucleus,Secreted,Cell membrane

Background

Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, aryl hydrocarbon (Ah) receptor, and some kinases. Studies have shown that murine HSP90 exists as two forms, HSP84 and HSP86, coded by related but separate genes, with 86% amino acid sequence conservation. These forms are analogous to the two forms of human HSP90, HSP89 alpha and HSP89 beta. In an unstressed mouse fibroblast, the basal level of HSP84 is found to be double that of HSP86. However, after heat shock, HSP86 shows a greater increase. Studies also suggest that upon cellular differentiation, the level of HSP86, but not HSP84, decreases. HSP84 and HSP86, which may be subject to estrogenic regulation, have been found as components of the non-DNA binding form of mouse glucocorticoid receptor, but dissociated from the transformed DNA-binding form.

Database

Research Area

DATA IMAGES

Anti-Hsp90 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79247

GTX79247 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX79247 HSP90 beta antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-Hsp90 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79247

GTX79247 ICC/IF Image

ICC/IF analysis of U251 cells using GTX79247 HSP90 beta antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-Hsp90 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX79247

GTX79247 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX79247 HSP90 beta antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-Hsp90 beta antibody used in Western Blot (WB). GTX79247

GTX79247 WB Image

WB analysis of 50μg of the indicated whole cell lysates per well using GTX79247 HSP90 beta antibody.
Dilution : 1:1000

Anti-Hsp90 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79247

GTX79247 ICC/IF Image

ICC/IF analysis of HeLa and NIH3T3 cells using GTX79247 HSP90 beta antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

Anti-Hsp90 beta antibody used in Flow cytometry (FACS). GTX79247

GTX79247 FACS Image

FACS analysis of permeabilized, fixed HeLa cells using GTX79247 HSP90 beta antibody.
Blue histogram : Primary antibody
Black histogram : Isotype control antibody
Dilution : 10 μg/ml incubated for 1 hour on ice

Anti-Hsp90 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX79247

GTX79247 IHC-P Image

IHC-P analysis of human placenta tissue using GTX79247 HSP90 beta antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-Hsp90 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX79247

GTX79247 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX79247 HSP90 beta antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

Anti-Hsp90 beta antibody used in Western Blot (WB). GTX79247

GTX79247 WB Image

WB analysis of mouse HSP86 (Hsp90 alpha) or HSP84 (Hsp90 beta) and protein using GTX22928 Hsp90 alpha antibody (Lane 1) or GTX79247 HSP90 beta antibody (Lane 2).

Anti-Hsp90 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79247

GTX79247 ICC/IF Image

ICC/IF analysis of A2058 Cells using GTX79247 HSP90 beta antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC

Anti-Hsp90 beta antibody used in Immunoprecipitation (IP). GTX79247

GTX79247 IP Image

IP analysis of HeLa cell lysates using GTX79247 HSP90 beta antibody.
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:1000

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399