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HSPA4 antibody [N3C2], Internal

Anti-HSPA4 antibody [N3C2], Internal used in Western Blot (WB). GTX112607
Anti-HSPA4 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX112607
Anti-HSPA4 antibody [N3C2], Internal used in Western Blot (WB). GTX112607

Cat. No. GTX112607

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all HSPA4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Positive Control

Raji , K562 , NIH-3T3

Predict Reactivity

Mouse, Rat, Bovine, Dog, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSPA4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165014

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 4 , APG-2 , HEL-S-5a , HS24/P52 , HSPH2 , RY , hsp70 , hsp70RY

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-HSPA4 antibody [N3C2], Internal used in Western Blot (WB). GTX112607

GTX112607 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
B: K562
5% SDS PAGE
GTX112607 diluted at 1:1000

Anti-HSPA4 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX112607

GTX112607 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using HSPA4(GTX112607) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-HSPA4 antibody [N3C2], Internal used in Western Blot (WB). GTX112607

GTX112607 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA4 antibody [N3C2], Internal (GTX112607) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169