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HUS1 antibody

Anti-HUS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32662
Anti-HUS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32662
Anti-HUS1 antibody used in Immunoprecipitation (IP). GTX32662
Anti-HUS1 antibody used in Western Blot (WB). GTX32662

Cat. No. GTX32662

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399)
See all HUS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HUS1 (NP_004498.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HUS1 checkpoint clamp component , hHUS1

Cellular Localization

Nucleus,Cytoplasm, cytosol

Background

The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-HUS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32662

GTX32662 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32662 HUS1 antibody.
Blue : DAPI

Anti-HUS1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32662

GTX32662 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32662 HUS1 antibody.
Blue : DAPI

Anti-HUS1 antibody used in Immunoprecipitation (IP). GTX32662

GTX32662 IP Image

IP analysis of 293T cell lysate using GTX32662 HUS1 antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000

Anti-HUS1 antibody used in Western Blot (WB). GTX32662

GTX32662 WB Image

WB analysis of various sample lysates using GTX32662 HUS1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399