APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Daudi Cell Lysate(GTX23951)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
HUWE1 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human HUWE1.The immunogen is located within amino acids 4250 - 4300 of HUWE1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 , ARF-BP1 , HECTH9 , HSPC272 , Ib772 , LASU1 , MULE , URE-B1 , UREB1
Cellular Localization
Cytoplasm,Nucleus
Background
The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.
Database
Research Area
DATA IMAGES
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GTX85051 ICC/IF Image
ICC/IF analysis of Daudi cells using GTX85051 HUWE1 antibody.
Working concentration : 20 μg/ml
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GTX85051 ICC/IF Image
ICC/IF analysis of Daudi cells using GTX85051 HUWE1 antibody.
Working concentration : 5 μg/ml
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GTX85051 WB Image
WB analysis of Daudi cell lysate using GTX85051 HUWE1 antibody.
Working concentration : 1 μg/ml
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REFERENCE
There are currently no references for HUWE1 antibody (GTX85051). Be the first to share your publications with this product.
REVIEW
HUWE1 antibody
Cat. No. GTX85051
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Rating
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( Average 5 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Chen-I Hsu submitted on 10-Sep-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
RD (ATCC CCL-136)
Amount used :
60μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:2000, 4°C, 16Hr
Lane Description :
Lane1 : RD cell expressing shRNA targeting Luciferase
Lane2 : RD cell expressing shRNA targeting HUWE1 (TRCN0000332987)
Lane3 : RD cell expressing shRNA targeting HUWE1 (TRCN0000073304)
Lane4 : RD cell expressing shRNA targeting HUWE1 (TRCN0000073305)
