APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT1D6 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Heme oxygenase3 protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heme oxygenase 1 , HMOX1 , HMOX1D , HO1 , HSP32 , bK286B10 , Heme Oxygenase 1 , Heme Oxygenase1 , HO-1
Cellular Localization
Microsome,Endoplasmic reticulum membrane
Background
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Heme Oxygenase 1 antibody [AT1D6] used in Western Blot (WB). GTX57699](/templates/default/js/lightbox/images/loading.gif "Anti-Heme Oxygenase 1 antibody [AT1D6] used in Western Blot (WB). GTX57699")
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GTX57699 WB Image
WB analysis of various samples using GTX57699 Heme Oxygenase 1 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : A549 whole cell lysate
Lane 3 : 293T whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lane 5 : TF-1 cell lysat
Loading : 40 μg
Dilution : 1:500
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GTX57699 ICC/IF Image
ICC/IF analysis of A549 cells using GTX57699 Heme Oxygenase 1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57699 FACS Image
FACS analysis of HeLa cells using GTX57699 Heme Oxygenase 1 antibody.
Cell Number: 1 x 10⁶ cells
Red: Primary antibody
Antibody amount: 2-5 μg
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GTX57699 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57699 Heme Oxygenase 1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
|
REFERENCE
There are currently no references for Heme Oxygenase 1 antibody [AT1D6] (GTX57699). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Heme Oxygenase 1 antibody [AT1D6] (GTX57699). Be the first to share your experience with this product.
![Anti-Heme Oxygenase 1 antibody [AT1D6] used in Western Blot (WB). GTX57699](/upload/website/prouct_img/small/GTX57699/GTX57699_20200204_WB_w_23061123_539.webp "Anti-Heme Oxygenase 1 antibody [AT1D6] used in Western Blot (WB). GTX57699")
![Anti-Heme Oxygenase 1 antibody [AT1D6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57699](/upload/website/prouct_img/small/GTX57699/GTX57699_20200204_IF1_w_23061123_489.webp "Anti-Heme Oxygenase 1 antibody [AT1D6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57699")
![Anti-Heme Oxygenase 1 antibody [AT1D6] used in Flow cytometry (FACS). GTX57699](/upload/website/prouct_img/small/GTX57699/GTX57699_20200204_FC_w_23061123_778.webp "Anti-Heme Oxygenase 1 antibody [AT1D6] used in Flow cytometry (FACS). GTX57699")
![Anti-Heme Oxygenase 1 antibody [AT1D6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57699](/upload/website/prouct_img/small/GTX57699/GTX57699_20200204_IF2_w_23061123_219.webp "Anti-Heme Oxygenase 1 antibody [AT1D6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57699")
![Anti-Heme Oxygenase 1 antibody [AT1D6] used in Western Blot (WB). GTX57699](/upload/website/prouct_img/normal/GTX57699/GTX57699_20200204_WB_w_23061123_539.webp "Anti-Heme Oxygenase 1 antibody [AT1D6] used in Western Blot (WB). GTX57699")
![Anti-Heme Oxygenase 1 antibody [AT1D6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57699](/upload/website/prouct_img/normal/GTX57699/GTX57699_20200204_IF1_w_23061123_489.webp "Anti-Heme Oxygenase 1 antibody [AT1D6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57699")
![Anti-Heme Oxygenase 1 antibody [AT1D6] used in Flow cytometry (FACS). GTX57699](/upload/website/prouct_img/normal/GTX57699/GTX57699_20200204_FC_w_23061123_778.webp "Anti-Heme Oxygenase 1 antibody [AT1D6] used in Flow cytometry (FACS). GTX57699")
![Anti-Heme Oxygenase 1 antibody [AT1D6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57699](/upload/website/prouct_img/normal/GTX57699/GTX57699_20200204_IF2_w_23061123_219.webp "Anti-Heme Oxygenase 1 antibody [AT1D6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57699")