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Her2 / ErbB2 antibody

Anti-Her2 / ErbB2 antibody used in Western Blot (WB). GTX50425
Anti-Her2 / ErbB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX50425
Anti-Her2 / ErbB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50425
Anti-Her2 / ErbB2 antibody used in Western Blot (WB). GTX50425

Cat. No. GTX50425

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all Her2 / ErbB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

138 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa. 1246~1250 (P-E-Y-L-G) derived from human Her2 / ErbB2.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166735

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

erb-b2 receptor tyrosine kinase 2 , CD340 , HER-2 , HER-2/neu , HER2 , MLN 19 , NEU , NGL , TKR1

Cellular Localization

Nucleus

Background

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Her2 / ErbB2 antibody used in Western Blot (WB). GTX50425

GTX50425 WB Image

WB analysis of extracts from MCF and SKOV3 cells using GTX50425 Her2 / ErbB2 antibody.

Anti-Her2 / ErbB2 antibody used in IHC (Paraffin sections) (IHC-P). GTX50425

GTX50425 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50425 Her2 / ErbB2 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-Her2 / ErbB2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50425

GTX50425 ICC/IF Image

ICC/IF analysis of methanol-fixed MCF-7 cells using GTX50425 Her2 / ErbB2 antibody.

Anti-Her2 / ErbB2 antibody used in Western Blot (WB). GTX50425

GTX50425 WB Image

The data was published in the journal Oncotarget in 2018. PMID: 29765526

REFERENCE

Application Reference

Sauveur J et al. Pharmacol Res Perspect 2020; 8 (4) : e00617 Characterization of T-DM1-resistant breast cancer cells.
Sauveur J et al. Oncotarget 2018; 9 (30) : 21141-21155 Esophageal cancer cells resistant to T-DM1 display alterations in cell adhesion and the prostaglandin pathway.
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399