APPLICATION
Application Note
IHC-P: Use at a dilution of 1/50 for 10 min at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
WB: Use at an assay dependent dilution. Predicted molecular weight: 146.8 kDa.
Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
Positive Control
Breast carcinoma or skeletal muscle
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (C terminal).
Purification
Immunogen affinity purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
erb-b2 receptor tyrosine kinase 4 , ALS19 , HER4 , p180erbB4
Cellular Localization
Cell membrane , Mitochondrion
Background
This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX15137 IHC-P Image
IHC-P analysis of human breast carcinoma using GTX15137 Her4 / ErbB4 antibody.
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GTX15137 WB Image
WB analysis of A431 cell lysate using GTX15137 Her4 / ErbB4 antibody.
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REFERENCE
There are currently no references for Her4 / ErbB4 antibody (GTX15137). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Her4 / ErbB4 antibody (GTX15137). Be the first to share your experience with this product.