APPLICATION
Application Note
The antibody recognizes all four isoforms of Hexokinase (1~4) in recombinant protein. The antibody has been tested by ELISA, Western blot analysis and Immunofluorescence assay to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1,000.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human Hexokinase 1 (1-917aa) purified from E. coli
Purification
By protein-G affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
hexokinase 1 , HK , HK1-ta , HK1-tb , HK1-tc , HKD , HKI , HMSNR , HXK1 , RP79 , hexokinase
Cellular Localization
Mitochondrion outer membrane
Background
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016]
Database
Research Area
DATA IMAGES
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GTX53727 WB Image
WB analysis of (A) recombinant protein (20ng or 100ng) of Hexokinase four isoform and (B) the indicated lysates (20ug) using Hexokinase antibody at a dilution of 1:1,000.
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GTX53727 ICC/IF Image
ICC/IF analysis of HeLa cells using Hexokinase antibody at a dilution of 1:1,000 (green) and Hoechst 33342 (blue).
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REFERENCE
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REVIEW
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