GeneTex
United States (US)

Hexokinase II antibody

GTX111525_40688_IHC_19011507_790.jpg
GTX111525_40436_20170621_IFA_19011507_154.jpg
GTX111525_42649_20161129_IHC-P_R_19011507_786.jpg
GTX111525_40436_IHC_M_19011507_657.jpg
GTX111525_42648_20161129_IHC-P_R_18121410_324.jpg
GTX111525_40436_WB_M_brain_18121410_277.jpg
GTX111525_42648_20161129_IHC-P_R_2_18121410_626.jpg
GTX111525_42649_20161020_WB_18121410_866.jpg

Cat No. GTX111525

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , Mouse brain

Predict Reactivity

Zebrafish, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Hexokinase II. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp686M1669 antibody, HKII antibody, HXK2 antibody, HK2 antibody, HK II antibody, hexokinase type II antibody, muscle form hexokinase antibody, hexokinase-2, muscle antibody, hexokinase-2 antibody, hexokinase 2 antibody

Cellular Localization

Mitochondrion outer membrane (By similarity)

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX111525_40688_IHC_19011507_790.jpg

GTX111525 IHC-P Image

Hexokinase II antibody detects HK2 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Hexokinase II antibody (GTX111525) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX111525_40436_20170621_IFA_19011507_154.jpg

GTX111525 ICC/IF Image

Hexokinase II antibody detects Hexokinase II protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Hexokinase II protein stained by Hexokinase II antibody (GTX111525) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX111525_42649_20161129_IHC-P_R_19011507_786.jpg

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat stomach by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
Hexokinase II antibody (GTX111525) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX111525_40436_IHC_M_19011507_657.jpg

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytosol on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Hexokinase II antibody (GTX111525) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX111525_42648_20161129_IHC-P_R_18121410_324.jpg

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Hexokinase II antibody (GTX111525) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX111525_40436_WB_M_brain_18121410_277.jpg

GTX111525 WB Image

Sample (20 µg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX111525 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111525_42648_20161129_IHC-P_R_2_18121410_626.jpg

GTX111525 IHC-P Image

Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Hexokinase II antibody (GTX111525) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX111525_42649_20161020_WB_18121410_866.jpg

GTX111525 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase II antibody (GTX111525) diluted at 1:10000.

REFERENCE
REVIEW

Hexokinase II antibody Cat No. GTX111525

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 23-Oct-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : Placenta tissue lysate
Amount used : 60 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169