Histone H1.2 antibody

Name: Histone H1.2 antibody
Brand: GeneTex
SKU: GTX122561
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-Histone H1.2 antibody used in IHC (Paraffin sections) (IHC-P). GTX122561

Anti-Histone H1.2 antibody used in Western Blot (WB). GTX122561

Anti-Histone H1.2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122561

Cat. No. GTX122561

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all Histone H1.2 products

View product citations for antibody GTX122561 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

HeLa , HeLa nucleus

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Histone H1.2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162414

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

histone cluster 1 H1 family member c , H1.2 , H1C , H1F2 , H1s-1

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]

Database

Gene ID: 3006 HIST1H1C
UniProt: P16403 HIST1H1C

Research Area

DATA IMAGES

GTX122561 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Histone H1.2(GTX122561) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX122561 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
B: HeLa nucleus
12% SDS PAGE
GTX122561 diluted at 1:10000

GTX122561 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Histone H1.2(GTX122561) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX122561 WB Image

The data was published in the journal Front Oncol in 2020.PMID: 32435614

GTX122561 WB Image

The data was published in the journal Cell Res in 2018. PMID: 29844578

GTX122561 WB Image

The data was published in the journal Cell Res in 2018. PMID: 29844578

GTX122561 WB Image

The data was published in the journal Front Oncol in 2020.PMID: 32435614

GTX122561 WB Image

The data was published in the journal Front Oncol in 2020.PMID: 32435614

GTX122561 WB Image

The data was published in the journal Front Oncol in 2020.PMID: 32435614

GTX122561 WB Image

The data was published in the journal Front Oncol in 2020.PMID: 32435614

REFERENCE

Application Reference

Li YH et al. Front Oncol 2020; 10 : 567 MTA1 Promotes Hepatocellular Carcinoma Progression by Downregulation of DNA-PK-Mediated H1.2^T146 Phosphorylation.

Uggenti C et al. Nat Genet 2020; 52 (12) : 1364-1372 cGAS-mediated induction of type I interferon due to inborn errors of histone pre-mRNA processing.

Reactivity : Human

Xu H et al. PLoS Pathog 2019; 15 (12) : e1008228 Novel replisome-associated proteins at cellular replication forks in EBV-transformed B lymphocytes.

Li Z et al. Cell Res 2018; (Epub) Destabilization of linker histone H1.2 is essential for ATM activation and DNA damage repair.

Application : WB

Reactivity : Human

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