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Histone H2A.vS137ph (phospho Ser137) antibody

Anti-Histone H2A.vS137ph (phospho Ser137) antibody used in Western Blot (WB). GTX48733

Cat. No. GTX48733

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA, ChIP assay, EM, Multiplexing

Reactivity

 Drosophila
Package
50 μg ($399)
See all Histone H2A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:400-1:1600
ICC/IF Assay dependent
IHC-P 2 μg/mL
ELISA 1:29000-1:30000
ChIP assay Assay dependent
EM Assay dependent
Multiplexing Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Product Note

Reactivity occurs against Drosophila H2AvD pS137 protein and the antibody is specific for the phosphorylated form of the protein.   Reactivity with non-phosphorylated Drosophila H2AvD is minimal by ELISA.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Drosophila

Immunogen

Synthetic peptide corresponding to the C-Terminal region near amino acids 125-141 of Drosophila melanogaster (fruit fly) H2AvD protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Histone H2A variant , 5499 , CG5499 , Dmel\CG5499 , H2A , H2A.F/Z , H2A.V , H2A.X , H2A.Z , H2AV , H2AV_DROM , H2AX , H2AZ , H2Av , H2AvD , H2a.V , H2av , H2avD , HIS , HIS2AV , HIS2AVD , His , His2 , His2A , His2AV , His2AvD , HisH2Av , Hist , Hist2av , gamma-H2AV , gamma-H2Av , gamma-HIS2AV , gamma-His2Av , gammaH2AV , gammaH2Av , h2AvD , his , his2Av , l(3)05146 , l(3)810 , l(3)97Dd , l(3)L1602

Cellular Localization

Nucleus

Background

Variant histones H2A are synthesized throughout the cell cycle and are very different from classical S-phase regulated H2A. H2AvD is vital for viability, but the exact function of variant histones H2A is not known. H2A is a core component of the nucleosome, an octamer containing two molecules each of H2A, H2B, H3 and H4. The octamer wraps approximately 146 bp of DNA. HsAvD is expressed both maternally and zygotically and is found in embryos through to adults (female only). The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur. In mouse, H2AX "knock out" mice have an increased incidence of cancer.

Database

Research Area

DATA IMAGES

Anti-Histone H2A.vS137ph (phospho Ser137) antibody used in Western Blot (WB). GTX48733

GTX48733 WB Image

Western blot using GeneTex's affinity purified anti-histone H2AvD pS137 antibody shows detection of a band at ~15 kDa corresponding to phosphorylated H2AvD (lane 2 arrow-head). Lanes contain either mock-irradiated (lane 1) or 4000-RAD gamma irradiated (lane 2) Drosophila melanogaster (3rd instar) larvae brain WC lysate separated on by SDS-PAGE and transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:500. Washes and reaction with secondary antibody followed incubation. Use HRP conjugated Gt-a-Rabbit IgG [H&L] MX and ECL for detection.

REFERENCE

Application Reference

Katie Burbridge et al. Development 2021; 148 (8) Metabolically active and polyploid renal tissues rely on graded cytoprotection to drive developmental and homeostatic stress resilience.
Reactivity : Drosophila
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399