Histone H2B antibody

Name: Histone H2B antibody
Brand: GeneTex
SKU: GTX129565
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-Histone H2B antibody used in Western Blot (WB). GTX129565

Anti-Histone H2B antibody used in Immunoprecipitation (IP). GTX129565

Cat. No. GTX129565

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IP
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX129565 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:20000
IP	1:100-1:500

Not tested in other applications.

Positive Control

Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , 293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H2B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886033

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX129565 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Histone H2B antibody (GTX129565) diluted at 1:5000 and competitor's antibody (sc-10808) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX129565 IP Image

Immunoprecipitation of Histone H2B protein from HeLa whole cell extracts using 5 μg of Histone H2B antibody (GTX129565).
Western blot analysis was performed using Histone H2B antibody (GTX129565) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX129565 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody (GTX129565) diluted at 1:20000.

REFERENCE

Application Reference

Tsai CH et al. Biochim Biophys Acta Mol Cell Res 2019; 1867 (2) : 118564 Aza-PBHA, a potent histone deacetylase inhibitor, inhibits human gastric-cancer cell migration via PKC帢-mediated AHR-HDAC interactions.

Application : WB

Reactivity : Human

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