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Histone H3 antibody

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148
Anti-Histone H3 antibody used in Western Blot (WB). GTX122148
Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148
Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148
Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148
Anti-Histone H3 antibody used in Western Blot (WB). GTX122148
Anti-Histone H3 antibody used in Western Blot (WB). GTX122148
Anti-Histone H3 antibody used in ChIP assay (ChIP assay). GTX122148
Anti-Histone H3 antibody used in Immunoprecipitation (IP). GTX122148
Anti-Histone H3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122148

Cat No. GTX122148

Reference 23
5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Drosophila, Monkey, Fungi, Golden Syrian Hamster, Rice
Package
100 μl ($319),
25 μl ($169)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , PC-12 , Rat2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Nucleus

Research Area

DATA IMAGES

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148

GTX122148 IHC-P Image

Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Green: Histone H3 antibody (GTX122148) diluted at 1:500.

Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3 antibody used in Western Blot (WB). GTX122148

GTX122148 WB Image

Histone H3 antibody detects Histone H3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122148) diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148

GTX122148 IHC-P Image

Histone H3 antibody detects Histone H3 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H3 antibody (GTX122148) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148

GTX122148 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Histone H3(GTX122148) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148

GTX122148 IHC-P Image

Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Histone H3 antibody (GTX122148) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3 antibody used in Western Blot (WB). GTX122148

GTX122148 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122148) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3 antibody used in Western Blot (WB). GTX122148

GTX122148 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122148) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3 antibody used in ChIP assay (ChIP assay). GTX122148

GTX122148 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-Histone H3 antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.

Anti-Histone H3 antibody used in Immunoprecipitation (IP). GTX122148

GTX122148 IP Image

Histone H3 antibody immunoprecipitates Histone H3 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of Histone H3 by 3 μg of Histone H3 antibody (GTX122148) 15% SDS-PAGE The immunoprecipitated Histone H3 protein was detected by Histone H3 antibody (GTX122148) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Histone H3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122148

GTX122148 ICC/IF Image

Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Histone H3 protein stained by Histone H3 antibody (GTX122148) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Luc?a D?vila et al. Odontoestomatolog?a 2019; (Epub) Inmunoexpresi?n de biomarcadores Bax, Bcl-2, CD138, H3, Ki-67, MCM3 y p53 en liquen plano oral.
Yi Ding et al. bioRxiv 2019; (Epub) Novel regulators of nitric oxide signaling triggered by host perception in a plant pathogen.
Application : WB
Dedoni S et al. J Pharmacol Exp Ther 2019; 370 (3) : 490-503 Downregulation of TrkB Expression and Signaling by Valproic Acid and Other Histone Deacetylase Inhibitors.
Application : WB
Reactivity : Human
Li M et al. Oncogene 2019; (Epub) Mono-ADP-ribosylation of H3R117 traps 5mC hydroxylase TET1 to impair demethylation of tumor suppressor gene TFPI2.
Application : WB
Reactivity : Human
Lee PC et al. Cancer Cell 2018; 34 (6) : 954-969.e4 Targeting PKCδ as a Therapeutic Strategy against Heterogeneous Mechanisms of EGFR Inhibitor Resistance in EGFR-Mutant Lung Cancer.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

Histone H3 antibody Cat No. GTX122148

Rating
( Average 4 based on 5 users reviews)
Application
Western Blot(WB)
( Average 4 based on 5 users reviews)
Anti-Histone H3 antibody used in Western Blot (WB). GTX122148
Date : Anonymous submitted on 05-Oct-2019
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells
Amount used : 10 μg
Blocking : 2.5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Anti-Histone H3 antibody used in Western Blot (WB). GTX122148
Date : Anonymous submitted on 05-Oct-2019
Score :
Application Tested : WB
Sample Species : Golden Syrian Hamster
Sample : BHK21 cells
Amount used : 5 μg
Blocking : 2.5% non-fat milk, 25°C, 0.5Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Anti-Histone H3 antibody used in Western Blot (WB). GTX122148
Date : Anonymous submitted on 23-May-2014
Score :
Application Tested : WB
Sample Species : Rice
Sample : rice leaf tissue
Amount used : 50μg
Blocking : 5% milk in tris buffer, 4°C, 1Hr
Primary Antibody : 1:2500, 4°C, 16Hr
Anti-Histone H3 antibody used in Western Blot (WB). GTX122148
Date : Anonymous submitted on 10-Mar-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : primary adipocyte cells
Amount used : 10μg
Blocking : 5% nonfat milk in TBST(1xTBS, 0.1%Tween 20), 25°C, 3Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Anti-Histone H3 antibody used in Western Blot (WB). GTX122148
Date : Anonymous submitted on 22-Dec-2012
Score :
Application Tested : WB
Sample Species : Mk
Sample : Vero E6 cells
Amount used : 100μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Submit a Review
Application Reference
Luc?a D?vila et al. Odontoestomatolog?a 2019; (Epub) Inmunoexpresi?n de biomarcadores Bax, Bcl-2, CD138, H3, Ki-67, MCM3 y p53 en liquen plano oral.
Yi Ding et al. bioRxiv 2019; (Epub) Novel regulators of nitric oxide signaling triggered by host perception in a plant pathogen.
Application : WB
Dedoni S et al. J Pharmacol Exp Ther 2019; 370 (3):490-503 Downregulation of TrkB Expression and Signaling by Valproic Acid and Other Histone Deacetylase Inhibitors.
Application : WB
Reactivity : Human
Li M et al. Oncogene 2019; (Epub) Mono-ADP-ribosylation of H3R117 traps 5mC hydroxylase TET1 to impair demethylation of tumor suppressor gene TFPI2.
Application : WB
Reactivity : Human
Lee PC et al. Cancer Cell 2018; 34 (6):954-969.e4 Targeting PKCδ as a Therapeutic Strategy against Heterogeneous Mechanisms of EGFR Inhibitor Resistance in EGFR-Mutant Lung Cancer.
Application : WB
Reactivity : Human
Hao H et al. Nucleic Acids Res 2018; (Epub) N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication.
Application : WB
Reactivity : Human
Singh R et al. Sci Rep 2018; 8 (1):15633 A role for calcium in resistin transcriptional activation in diabetic hearts.
Takashina Y et al. Nutrients 2018; 10 (10) Sodium Citrate Increases Expression and Flux of Mg2+ Transport Carriers Mediated by Activation of MEK/ERK/c-Fos Pathway in Renal Tubular Epithelial Cells.
Application : WB
Reactivity : Rat
Lopez-Charcas O et al. Sci Rep 2018; 8 (1):12995 The invasiveness of human cervical cancer associated to the function of NaV1.6 channels is mediated by MMP-2 activity.
Application : WB
Reactivity : Human
Fang P et al. Mol Cancer Res 2018; (Epub) Olaparib-induced Adaptive Response is Disrupted by FOXM1 Targeting which Enhances Sensitivity to PARP Inhibition.
Application : WB
Reactivity : Human
Lin SC et al. Oncol Rep 2018; 39 (2):703-710 Glycyrrhizic acid induces human MDA-MB-231 breast cancer cell death and autophagy via the ROS-mitochondrial pathway.
Application : WB
Hollingworth R et al. J Virol 2017; 91 (22) Localization of Double-Strand Break Repair Proteins to Viral Replication Compartments following Lytic Reactivation of Kaposi's Sarcoma-Associated Herpesvirus.
Chettri P et al. Mol Microbiol 2017; (Epub) Chromatin-level regulation of the fragmented dothistromin gene cluster in the forest pathogen Dothistroma septosporum.
Application : WB
Reactivity : Fungi
Yang WS et al. PLoS Pathog 2017; 13 (2):e1006216 SUMO modification of a heterochromatin histone demethylase JMJD2A enables viral gene transactivation and viral replication.
Application : WB
Reactivity : Human
Tenreiro P et al. Anal Biochem 2016; 517:43108 Comparison of simple sucrose and percoll based methodologies for synaptosome enrichment.
Application : WB
Reactivity : Rat
Shih CC et al. PLoS One 2016; 11 (9):e0163690 Procainamide Inhibits DNA Methylation and Alleviates Multiple Organ Dysfunction in Rats with Endotoxic Shock.
Application : WB
Reactivity : Rat
Horke S et al. Infect Immun 2015; 83 (9):3369-80 Novel Paraoxonase 2-Dependent Mechanism Mediating the Biological Effects of the Pseudomonas aeruginosa Quorum-Sensing Molecule N-(3-Oxo-Dodecanoyl)-L-Homoserine Lactone.
Application : WB
Reactivity : Human
Hii HP et al. PLoS One 2015; 10 (7):e0134492 Distinct Patterns of Wnt3a and Wnt5a Signaling Pathway in the Lung from Rats with Endotoxic Shock.
Application : WB
Reactivity : Rat
Li PT et al. Int J Mol Sci 2015; 16 (10):23994-4010 Increased Histone Deacetylase Activity Involved in the Suppressed Invasion of Cancer Cells Survived from ALA-Mediated Photodynamic Treatment.
Application : WB
Reactivity : Human
Li CJ et al. Sci Rep 2015; (Epub) Synergistic protection of N-acetylcysteine and ascorbic acid 2-phosphate on human mesenchymal stem cells against mitoptosis, necroptosis and apoptosis.
Application : WB
Reactivity : Human
Huang YC et al. PLoS Genet 2014; 10 (11):e1004760 The COP9 Signalosome Converts Temporal Hormone Signaling to Spatial Restriction on Neural Competence.
Application : ICC/IF
Reactivity : Drosophila
Lee KH et al. Sci Rep 2014; 4:6394 A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.
Application : WB
Reactivity : Human
Lee YR et al. J Biomed Sci 2012; 19:9 Reversine suppresses oral squamous cell carcinoma via cell cycle arrest and concomitantly apoptosis and autophagy.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169