GeneTex
  • Country / Location Selection

United States (US)

Histone H3 antibody

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148
Anti-Histone H3 antibody used in Western Blot (WB). GTX122148
Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148
Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148
Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148
Anti-Histone H3 antibody used in Western Blot (WB). GTX122148
Anti-Histone H3 antibody used in Western Blot (WB). GTX122148
Anti-Histone H3 antibody used in ChIP assay (ChIP assay). GTX122148
Anti-Histone H3 antibody used in Immunoprecipitation (IP). GTX122148
Anti-Histone H3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122148

Cat No. GTX122148

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human, Mouse, Rat, Drosophila, Monkey, Fungi, Rice
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , PC-12 , Rat2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Nucleus

Research Area

DATA IMAGES
Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148

GTX122148 IHC-P Image

Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Green: Histone H3 antibody (GTX122148) diluted at 1:500.

Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3 antibody used in Western Blot (WB). GTX122148

GTX122148 WB Image

Histone H3 antibody detects Histone H3 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122148) diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148

GTX122148 IHC-P Image

Histone H3 antibody detects Histone H3 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H3 antibody (GTX122148) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148

GTX122148 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Histone H3(GTX122148) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3 antibody used in IHC (Paraffin sections) (IHC-P). GTX122148

GTX122148 IHC-P Image

Histone H3 antibody detects Histone H3 protein at nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Histone H3 antibody (GTX122148) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3 antibody used in Western Blot (WB). GTX122148

GTX122148 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122148) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3 antibody used in Western Blot (WB). GTX122148

GTX122148 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (GTX122148) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3 antibody used in ChIP assay (ChIP assay). GTX122148

GTX122148 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-Histone H3 antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.

Anti-Histone H3 antibody used in Immunoprecipitation (IP). GTX122148

GTX122148 IP Image

Histone H3 antibody immunoprecipitates Histone H3 protein in IP experiments. IP Sample: Raji whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of Histone H3 by 3 μg of Histone H3 antibody (GTX122148) 15% SDS-PAGE The immunoprecipitated Histone H3 protein was detected by Histone H3 antibody (GTX122148) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Histone H3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX122148

GTX122148 ICC/IF Image

Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Histone H3 protein stained by Histone H3 antibody (GTX122148) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
REVIEW

Histone H3 antibody Cat No. GTX122148

Rating ( Average 4 based on 3 users reviews)
Application
Western Blot(WB) ( Average 4 based on 3 users reviews)
Date : Anonymous submitted on 23-May-2014
Score :
Application Tested : WB
Sample Species : Rice
Sample : rice leaf tissue
Amount used : 50μg
Blocking : 5% milk in tris buffer, 4°C, 1Hr
Primary Antibody : 1:2500, 4°C, overnightHr
Date : Anonymous submitted on 10-Mar-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : primary adipocyte cells
Amount used : 10μg
Blocking : 5% nonfat milk in TBST(1xTBS, 0.1%Tween 20), 25°C, 3Hr
Primary Antibody : 1:2000, 4°C, overnightHr
Date : Anonymous submitted on 22-Dec-2012
Score :
Application Tested : WB
Sample Species : Mk
Sample : Vero E6 cells
Amount used : 100μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169