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Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade

Anti-Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade used in Western Blot (WB). GTX60815
Anti-Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60815
Anti-Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60815
Anti-Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade used in ELISA (ELISA). GTX60815
Anti-Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade used in Dot blot (Dot). GTX60815
Anti-Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60815

Cat. No. GTX60815

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, Dot, ELISA, ChIP assay, ChIP-seq

Reactivity

 Human, Mouse, Rat, Arabidopsis thaliana
Package
50 μg ($529)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1,000
ICC/IF 1:500
Dot 1:20,000
ELISA 1:500
ChIP assay 0.5-5 μg
ChIP-seq Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The region of histone H3 containing the acetylated lysine 27 (H3K27ac), using a KLH-conjugated synthetic peptide.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2888004

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade used in Western Blot (WB). GTX60815

GTX60815 WB Image

WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using GTX60815 Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade.
Dilution : 1:1,000

Anti-Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60815

GTX60815 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60815 Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:500

Anti-Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60815

GTX60815 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ HeLa cells using GTX60815 Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade. A titration consisting of 0.2, 0.5, 1 and 2 μg of antibody per ChIP experiment was analyzed. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active GAPDH and EIF4A2 genes, used as positive controls, and for the coding regions of the inactive MB and MYT1 genes, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Anti-Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade used in ELISA (ELISA). GTX60815

GTX60815 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60815 Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade.

Anti-Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade used in Dot blot (Dot). GTX60815

GTX60815 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H3K27 using GTX60815 Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade.
Dilution : 1:20,000

Anti-Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60815

GTX60815 ChIP assay Image

ChIP analysis of sheared chromatin from 1x10⁵ K562 cells using GTX60815 Histone H3K27ac (Acetyl Lys27) antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2A shows the peak distribution along the complete human X-chromosome. Figure 2 B and C show the peak distribution in two regions surrounding the EIF4A2 and GAPDH positive control genes, respectively. The position of the PCR amplicon, used for validating the ChIP assay is indicated with an arrow.
Antibody amount : 1μg

REFERENCE

Application Reference

Kitagawa Y et al. iScience 2023; 26 (10) : 107893 ZEB2 and MEIS1 independently contribute to hematopoiesis via early hematopoietic enhancer activation.
Y Kitagawa et al. ISCIENCE 2023; S2589-0042 (23) : 01970-01973 ZEB2 and MEIS1 independently contribute to hematopoiesis via early hematopoietic enhancer activation
Application : ChIP assay
Nakatsukasa H et al. Int Immunol 2019; (Epub) Loss of TET proteins in regulatory T cells promotes abnormal proliferation, Foxp3 destabilization, and IL-17 expression.
Application : ChIP-seq
Reactivity : Mouse
Submit a Reference

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Package List Price ($)
$ 529