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Histone H4 antibody

Anti-Histone H4 antibody used in IHC (Paraffin sections) (IHC-P). GTX129560
Anti-Histone H4 antibody used in IHC (Paraffin sections) (IHC-P). GTX129560
Anti-Histone H4 antibody used in Western Blot (WB). GTX129560
Anti-Histone H4 antibody used in Western Blot (WB). GTX129560
Anti-Histone H4 antibody used in IHC (Paraffin sections) (IHC-P). GTX129560
Anti-Histone H4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129560
Anti-Histone H4 antibody used in IHC (Paraffin sections) (IHC-P). GTX129560

Cat. No. GTX129560

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Histone H4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:5000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

293T , A431 , HeLa , HepG2 , zebrafish brain , eye

Predict Reactivity

Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886030

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H4 antibody used in IHC (Paraffin sections) (IHC-P). GTX129560

GTX129560 IHC-P Image

Histone H4 antibody detects Histone H4 protein at nucleus on rat brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain stem.
Histone H4 antibody (GTX129560) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H4 antibody used in IHC (Paraffin sections) (IHC-P). GTX129560

GTX129560 IHC-P Image

Histone H4 antibody detects Histone H4 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Histone H4 antibody (GTX129560) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H4 antibody used in Western Blot (WB). GTX129560

GTX129560 WB Image

Histone H4 antibody detects Histone H4 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody (GTX129560) diluted at a dilution of 1:5000.

Anti-Histone H4 antibody used in Western Blot (WB). GTX129560

GTX129560 WB Image

Various tissue extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody (GTX129560) diluted at 1:1000.

Anti-Histone H4 antibody used in IHC (Paraffin sections) (IHC-P). GTX129560

GTX129560 IHC-P Image

Histone H4 antibody detects Histone H4 protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Histone H4 antibody (GTX129560) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129560

GTX129560 ICC/IF Image

Histone H4 antibody detects Histone H4 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4 protein stained by Histone H4 antibody (GTX129560) diluted at 1:5000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Anti-Histone H4 antibody used in IHC (Paraffin sections) (IHC-P). GTX129560

GTX129560 IHC-P Image

Histone H4 antibody detects Histone H4 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Histone H4 antibody (GTX129560) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Chomiak AA et al. iScience 2022; 25 (6) : 104354 Nde1 is required for heterochromatin compaction and stability in neocortical neurons.
Application : WB
Reactivity : Mouse
Yan Guo et al. iScience 2022; 25 (7) : 104519 Histone H2A ubiquitination resulting from Brap loss of function connects multiple aging hallmarks and accelerates neurodegeneration.
Liu MK et al. Int J Mol Sci 2016; 17 (6) Topoisomerase II Inhibitors Can Enhance Baculovirus-Mediated Gene Expression in Mammalian Cells through the DNA Damage Response.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169