Histone H4 antibody

Name: Histone H4 antibody
Brand: GeneTex
SKU: GTX129561
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-Histone H4 antibody used in IHC (Paraffin sections) (IHC-P). GTX129561

Anti-Histone H4 antibody used in Western Blot (WB). GTX129561

Anti-Histone H4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129561

Cat. No. GTX129561

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Drosophila
Package	100 μl ($399), 25 μl ($169)

See all Histone H4 products

Datasheet
Document

View product citations for antibody GTX129561 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:10000
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Predict Reactivity

Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886031

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX129561 IHC-P Image

Histone H4 antibody detects Histone H4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Histone H4 stained by Histone H4 antibody (GTX129561) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX129561 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody (GTX129561) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX129561 ICC/IF Image

Histone H4 antibody detects Histone H4 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4 stained by Histone H4 antibody (GTX129561) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

GTX129561 IHC-P Image

Histone H4 antibody detects Histone H4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Histone H4 stained by Histone H4 antibody (GTX129561) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX129561 IHC-P Image

Histone H4 antibody detects Histone H4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Histone H4 stained by Histone H4 antibody (GTX129561) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX129561 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody (GTX129561) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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REVIEW