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Histone H4K5ac (acetyl Lys5) antibody [GT1138]

Recombinant antibody Orthogonal
Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Western Blot (WB). GTX00775
Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Western Blot (WB). GTX00775
Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775
Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775
Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775
Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775
Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775
Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775

Cat. No. GTX00775

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1138

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Histone H4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from hmuan Histone H4 (acetyl K5)

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Western Blot (WB). GTX00775

GTX00775 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4K5ac (acetyl Lys5) antibody [GT1138] (GTX00775) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Western Blot (WB). GTX00775

GTX00775 WB Image

WB analysis of HeLa and C6 whole cell lysates using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Dilution : 1:1000
Loading : 25μg

Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775

GTX00775 ICC/IF Image

Orthogonal

ICC/IF analysis of C6 cells treated with/without TSA (1 μM at 37ºC for 18 hours) using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Orange: Primary antibody
Blue: DAPI

Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775

GTX00775 ICC/IF Image

Orthogonal

ICC/IF analysis of U2OS cells treated with/without TSA (1 μM at 37ºC for 18 hours) using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Orange: Primary antibody
Blue: DAPI

Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00775

GTX00775 ICC/IF Image

Orthogonal

ICC/IF analysis of NIH3T3 cells treated with/without TSA (1 μM at 37ºC for 18 hours) using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Orange: Primary antibody
Blue: DAPI

Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775

GTX00775 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Dlution : 1:100

Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775

GTX00775 IHC-P Image

IHC-P analysis of mouse kidney tissue section using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Dlution : 1:100

Anti-Histone H4K5ac (acetyl Lys5) antibody [GT1138] used in IHC (Paraffin sections) (IHC-P). GTX00775

GTX00775 IHC-P Image

IHC-P analysis of hmuan appendix tissue section using GTX00775 Histone H4K5ac (acetyl Lys5) antibody [GT1138].
Dlution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399