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Homer3 antibody

Anti-Homer3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115242
Anti-Homer3 antibody used in Western Blot (WB). GTX115242
Anti-Homer3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115242
Anti-Homer3 antibody used in IHC (Paraffin sections) (IHC-P). GTX115242
Anti-Homer3 antibody used in Western Blot (WB). GTX115242

Cat. No. GTX115242

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all HOMER3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Mouse, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Homer3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621284

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

homer scaffold protein 3 , HOMER-3 , VESL3

Cellular Localization

Cytoplasm , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density , synapse

Background

This gene encodes a member of the HOMER family of postsynaptic density scaffolding proteins that share a similar domain structure consisting of an N-terminal Enabled/vasodilator-stimulated phosphoprotein homology 1 domain which mediates protein-protein interactions, and a carboxy-terminal coiled-coil domain and two leucine zipper motifs that are involved in self-oligomerization. The encoded protein binds numerous other proteins including group I metabotropic glutamate receptors, inositol 1,4,5-trisphosphate receptors and amyloid precursor proteins and has been implicated in diverse biological functions such as neuronal signaling, T-cell activation and trafficking of amyloid beta peptides. Alternative splicing results in multiple transcript variants.

Database

Research Area

DATA IMAGES

Anti-Homer3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115242

GTX115242 ICC/IF Image

Homer3 antibody detects Homer3 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Homer3 protein stained by Homer3 antibody (GTX115242) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Homer3 antibody used in Western Blot (WB). GTX115242

GTX115242 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
10% SDS PAGE
GTX115242 diluted at 1:1000

Anti-Homer3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115242

GTX115242 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Homer3(GTX115242) antibody at 1:500 dilution.

Anti-Homer3 antibody used in IHC (Paraffin sections) (IHC-P). GTX115242

GTX115242 IHC-P Image

Homer3 antibody detects Homer3 protein at cytoplasm on human Papillary adenocarcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded Papillary adenocarcinoma .
Homer3 antibody (GTX115242) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Homer3 antibody used in Western Blot (WB). GTX115242

GTX115242 WB Image

The data was published in the journal Cell Death Dis in 2018.PMID: 29497031

REFERENCE

Application Reference

Chen S et al. Cell Death Dis 2018; 9 (3) : 347 WW domain-binding protein 2 acts as an oncogene by modulating the activity of the glycolytic enzyme ENO1 in glioma.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169