APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:2000 |
| 1:100 |
| 1:50 |
| 1:100 |
Not tested in other applications.
Product Note
This antibody is able to recognize Hsp70 protein , which includes HSPA1A and HSPA1B protein.
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human recombinant protein of human Hsp70 produced in HEK293T cell.
Purification
Purified by affinity chromatography
From tissue culture supernatant
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70
Cellular Localization
Cytoplasm,Nucleus
Background
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.
Database
Research Area
DATA IMAGES
![Anti-Hsp70 antibody [3C6] used in Western Blot (WB). GTX84338](/templates/default/js/lightbox/images/loading.gif "Anti-Hsp70 antibody [3C6] used in Western Blot (WB). GTX84338")
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GTX84338 WB Image
WB analysis of various cell lines using GTX84338 Hsp70 antibody [3C6].
Loading : 35 ug per lane
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GTX84338 WB Image
WB analysis of HEK293T cells transfected with Hsp70 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84338 Hsp70 antibody [3C6].
Loading : 5 ug per lane
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GTX84338 FACS Image
FACS analysis of HeLa cells using GTX84338 Hsp70 antibody [3C6].
Red : Primary antibody
Blue : Negative control antibody
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GTX84338 IHC-P Image
IHC-P analysis of human colon adenocarcinoma tissue using GTX84338 Hsp70 antibody [3C6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
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GTX84338 ICC/IF Image
ICC/IF analysis of COS7 cells transiently transfected with Hsp70 plasmid using GTX84338 Hsp70 antibody [3C6].
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GTX84338 IHC-P Image
IHC-P analysis of human liver tissue using GTX84338 Hsp70 antibody [3C6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
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REFERENCE
There are currently no references for Hsp70 antibody [3C6] (GTX84338). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Hsp70 antibody [3C6] (GTX84338). Be the first to share your experience with this product.
![Anti-Hsp70 antibody [3C6] used in Western Blot (WB). GTX84338](/upload/website/prouct_img/small/GTX84338/GTX84338_4288_WB_w_23061420_901.webp "Anti-Hsp70 antibody [3C6] used in Western Blot (WB). GTX84338")
![Anti-Hsp70 antibody [3C6] used in Western Blot (WB). GTX84338](/upload/website/prouct_img/small/GTX84338/GTX84338_4287_WB_w_23061420_498.webp "Anti-Hsp70 antibody [3C6] used in Western Blot (WB). GTX84338")
![Anti-Hsp70 antibody [3C6] used in Flow cytometry (FACS). GTX84338](/upload/website/prouct_img/small/GTX84338/GTX84338_367_FACS_w_23061420_535.webp "Anti-Hsp70 antibody [3C6] used in Flow cytometry (FACS). GTX84338")
![Anti-Hsp70 antibody [3C6] used in IHC (Paraffin sections) (IHC-P). GTX84338](/upload/website/prouct_img/small/GTX84338/GTX84338_2630_IHC-P_w_23061420_217.webp "Anti-Hsp70 antibody [3C6] used in IHC (Paraffin sections) (IHC-P). GTX84338")
![Anti-Hsp70 antibody [3C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84338](/upload/website/prouct_img/small/GTX84338/GTX84338_1042_ICCIF_w_23061420_652.webp "Anti-Hsp70 antibody [3C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84338")
![Anti-Hsp70 antibody [3C6] used in IHC (Paraffin sections) (IHC-P). GTX84338](/upload/website/prouct_img/small/GTX84338/GTX84338_2631_IHC-P_w_23061420_359.webp "Anti-Hsp70 antibody [3C6] used in IHC (Paraffin sections) (IHC-P). GTX84338")
![Anti-Hsp70 antibody [3C6] used in Western Blot (WB). GTX84338](/upload/website/prouct_img/normal/GTX84338/GTX84338_4288_WB_w_23061420_901.webp "Anti-Hsp70 antibody [3C6] used in Western Blot (WB). GTX84338")
![Anti-Hsp70 antibody [3C6] used in Western Blot (WB). GTX84338](/upload/website/prouct_img/normal/GTX84338/GTX84338_4287_WB_w_23061420_498.webp "Anti-Hsp70 antibody [3C6] used in Western Blot (WB). GTX84338")
![Anti-Hsp70 antibody [3C6] used in Flow cytometry (FACS). GTX84338](/upload/website/prouct_img/normal/GTX84338/GTX84338_367_FACS_w_23061420_535.webp "Anti-Hsp70 antibody [3C6] used in Flow cytometry (FACS). GTX84338")
![Anti-Hsp70 antibody [3C6] used in IHC (Paraffin sections) (IHC-P). GTX84338](/upload/website/prouct_img/normal/GTX84338/GTX84338_2630_IHC-P_w_23061420_217.webp "Anti-Hsp70 antibody [3C6] used in IHC (Paraffin sections) (IHC-P). GTX84338")
![Anti-Hsp70 antibody [3C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84338](/upload/website/prouct_img/normal/GTX84338/GTX84338_1042_ICCIF_w_23061420_652.webp "Anti-Hsp70 antibody [3C6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84338")
![Anti-Hsp70 antibody [3C6] used in IHC (Paraffin sections) (IHC-P). GTX84338](/upload/website/prouct_img/normal/GTX84338/GTX84338_2631_IHC-P_w_23061420_359.webp "Anti-Hsp70 antibody [3C6] used in IHC (Paraffin sections) (IHC-P). GTX84338")