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Human Interferon gamma protein, His tag (active)

Human Interferon gamma protein, His tag (active). GTX00084-pro
Human Interferon gamma protein, His tag (active). GTX00084-pro
Human Interferon gamma protein, His tag (active). GTX00084-pro

Cat. No. GTX00084-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($219)
See all Interferon gamma protein products

APPLICATION

Application Note

IFN-γ is a dimerized soluble cytokine that is the only member of the type II class of interferons. The importance of IFNγ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. As reported, IFNγ is an important activator of human monocytic THP-1 cells. Therefore, THP-1 cells were incubated in RPMI-1640 with various concentration of IFN-γ, then cells were observed by inverted microscope everyday. After stimulated with IFN-γ(5 ng/ml) for 5 days, morphological changes occured in THP-1 cells which displayed the shape of fusiform or polygon and were more likely to adhere.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Gln24~Gln166 (NP_000610.2)

Expression System

HEK293 cells

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interferon gamma , IFG , IFI

Background

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Human Interferon gamma protein, His tag (active). GTX00084-pro

GTX00084-pro Functional Assay Image

Activation effect of GTX00084-pro Human Interferon gamma protein (active).
(A) Unstimulated THP-1 cells cultured in RPMI-1640.
(B) THP-1 cells cultured in RPMI-1640, stimulated with 2 ng/ml IFN gamma for 5 days.

Human Interferon gamma protein, His tag (active). GTX00084-pro

GTX00084-pro Functional Assay Image

ELISA detection of iNOS expression from RAW 246.7 cells stimulated by GTX00084-pro Human Interferon gamma protein (active) at 2 ng/ml for 24hrs.

Human Interferon gamma protein, His tag (active). GTX00084-pro

GTX00084-pro Functional Assay Image

Activation effect of GTX00084-pro Human Interferon gamma protein (active).
(A) Unstimulated Raw 246.7 cells cultured in DMEM (negative control).
(B) Raw 264.7 cells cultured in DMEM, stimulated with IFNg.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 219