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Human Leptin protein (active)

Human Leptin protein (active). GTX00170-pro
Human Leptin protein (active). GTX00170-pro
Human Leptin protein (active). GTX00170-pro

Cat No. GTX00170-pro

Application Functional Assay
Species Human
Package
10 μg ($199)
APPLICATION

Application Note

Leptin is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Many of leptin'Seffects are mediated through neuropeptide- containing neurons and neuropeptide receptors in the hypothalamus. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Besides, Hexokinase 3, White Cell (HK3) has been identified as an interactor of LEP, thus a binding ELISA assay was conducted to detect the interaction of recombinant human LEP and recombinant human HK3. Briefly, LEP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to HK3-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-LEPpAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of of LEP and HK3 was in a dose dependent manner.

Observed MW

46 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Val22~Cys167 (NP_000221.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

leptin,LEPD,OB,OBS

Background

This gene encodes a protein that is secreted by white adipocytes into the circulation and plays a major role in the regulation of energy homeostasis. Circulating leptin binds to the leptin receptor in the brain, which activates downstream signaling pathways that inhibit feeding and promote energy expenditure. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation and wound healing. Mutations in this gene and its regulatory regions cause severe obesity and morbid obesity with hypogonadism in human patients. A mutation in this gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES
Human Leptin protein (active). GTX00170-pro

GTX00170-pro Functional Assay Image

Functional ELISA analysis of GTX00170-pro Human Leptin protein (active) which can bind immobilized HK3 protein.

Human Leptin protein (active). GTX00170-pro

GTX00170-pro Image

WB analysis of GTX00170-pro Human Leptin protein (active).

Human Leptin protein (active). GTX00170-pro

GTX00170-pro Image

SDS-PAGE analysis of GTX00170-pro Human Leptin protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 199