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IARS antibody

Anti-IARS antibody used in Immunoprecipitation (IP). GTX131733
Anti-IARS antibody used in IHC (Paraffin sections) (IHC-P). GTX131733
Anti-IARS antibody used in Western Blot (WB). GTX131733

Cat. No. GTX131733

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all IARS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

144 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IARS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886518

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

isoleucyl-tRNA synthetase , GRIDHH , IARS1 , ILERS , ILRS , IRS , PRO0785

Cellular Localization

Cytoplasm

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Two alternatively spliced variants have been isolated that represent alternate 5' UTRs. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IARS antibody used in Immunoprecipitation (IP). GTX131733

GTX131733 IP Image

Immunoprecipitation of IARS protein from 293T whole cell extracts using 5 μg of IARS antibody (GTX131733).
Western blot analysis was performed using IARS antibody (GTX131733) diluted at 1:2500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-IARS antibody used in IHC (Paraffin sections) (IHC-P). GTX131733

GTX131733 IHC-P Image

IARS antibody detects IARS protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
IARS antibody (GTX131733) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-IARS antibody used in Western Blot (WB). GTX131733

GTX131733 WB Image

IARS antibody detects IARS protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IARS antibody (GTX131733) diluted at 1:4000.

REFERENCE

Application Reference

Watanabe M et al. Int J Mol Sci 2023; 24 (8) Molecular and Pathological Analyses of IARS1-Deficient Mice: An IARS Disorder Model.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169