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IARS2 antibody

Anti-IARS2 antibody used in Western Blot (WB). GTX87187
Anti-IARS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87187
Anti-IARS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX87187

Cat. No. GTX87187

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Monkey
Package
100 μg ($409)
See all IARS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
IHC-P 1:50~1:100
Not tested in other applications.

Calculated MW

114 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human IARS2 (71-120).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CAGSSS , IARS2 , ILERS , isoleucyltRNA synthetase 2, mitochondrial , isoleucyl-tRNA synthetase 2, mitochondrial

Cellular Localization

Mitochondrion matrix

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-IARS2 antibody used in Western Blot (WB). GTX87187

GTX87187 WB Image

WB analysis of RAW264.7 and COS7 cell lysates using GTX87187 IARS2 antibody. The lane on the right is blocked with the synthesized peptide.

Anti-IARS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87187

GTX87187 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX87187 IARS2 antibody. The picture on the right is blocked with the synthesized peptide.

Anti-IARS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX87187

GTX87187 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX87187 IARS2 antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409