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ID3 antibody [7B6]

Anti-ID3 antibody [7B6] used in Western Blot (WB). GTX84327
Anti-ID3 antibody [7B6] used in IHC (Paraffin sections) (IHC-P). GTX84327
Anti-ID3 antibody [7B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84327
Anti-ID3 antibody [7B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84327
Anti-ID3 antibody [7B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84327

Cat. No. GTX84327

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7B6

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($519)
See all ID3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human ID3 (NP_002158) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inhibitor of DNA binding 3, HLH protein , HEIR-1 , bHLHb25

Cellular Localization

Nucleus

Background

ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors.

Database

Research Area

DATA IMAGES

Anti-ID3 antibody [7B6] used in Western Blot (WB). GTX84327

GTX84327 WB Image

WB analysis of HEK293T cells transfected with ID3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84327 ID3 antibody [7B6].
Loading : 5 ug per lane

Anti-ID3 antibody [7B6] used in IHC (Paraffin sections) (IHC-P). GTX84327

GTX84327 IHC-P Image

IHC-P analysis of kidney tissue using GTX84327 ID3 antibody [7B6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-ID3 antibody [7B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84327

GTX84327 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX84327 ID3 antibody [7B6].

Anti-ID3 antibody [7B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84327

GTX84327 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with ID3 plasmid using GTX84327 ID3 antibody [7B6].

Anti-ID3 antibody [7B6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84327

GTX84327 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX84327 ID3 antibody [7B6].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519