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IKB alpha antibody [6A920]

Orthogonal
Anti-IKB alpha antibody [6A920] used in IHC (Paraffin sections) (IHC-P). GTX12134
Anti-IKB alpha antibody [6A920] used in Western Blot (WB). GTX12134
Anti-IKB alpha antibody [6A920] used in Flow cytometry (FACS). GTX12134
Anti-IKB alpha antibody [6A920] used in Western Blot (WB). GTX12134
Anti-IKB alpha antibody [6A920] used in Western Blot (WB). GTX12134
Anti-IKB alpha antibody [6A920] used in Western Blot (WB). GTX12134

Cat. No. GTX12134

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6A920

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IP, MS

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all IKB alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/ml
ICC/IF 1:20 - 1:1000
IHC-P 1:100
FACS 0.25 - 1 μg / 10⁶ cells
IP 1 μg/ml
MS Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A recombinant protein corresponding to amino acid residues 32-291 of human IkBa was used as immunogen.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NFKB inhibitor alpha , EDAID2 , IKBA , MAD-3 , NFKBI

Cellular Localization

Cytoplasm,Nucleus

Background

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-IKB alpha antibody [6A920] used in IHC (Paraffin sections) (IHC-P). GTX12134

GTX12134 IHC-P Image

IHC-P analysis of human breast tissue using GTX12134 IKB alpha antibody [6A920].
Dilution : 5 μg/ml
Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0

Anti-IKB alpha antibody [6A920] used in Western Blot (WB). GTX12134

GTX12134 WB Image

WB analysis of Jurkat, LNCaP, PC-3, HeLa, NIH3T3 cell lysates using GTX12134 IKB alpha antibody [6A920].
Dilution : 0.5 μg/ml

Anti-IKB alpha antibody [6A920] used in Flow cytometry (FACS). GTX12134

GTX12134 FACS Image

FACS (Intracellular staining) analysis of THP-1 cells using GTX12134 IKB alpha antibody [6A920].
Red : Primary antibody
Green : isotype control
Shaded histogram : cell only
Antibody amount : 0.25μg/10^6 cells

Anti-IKB alpha antibody [6A920] used in Western Blot (WB). GTX12134

GTX12134 WB Image

Orthogonal

WB analysis of 100 μg/ml ALLN* (N-Acetyl-Leu-Leu-Norleucinal) treated Jurkat cell followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3) using GTX12134 IKB alpha antibody [6A920] and IKB alpha phospho-specific antibody.
*ALLN (N-Acetyl-Leu-Leu-Norleucinal) : Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation

Anti-IKB alpha antibody [6A920] used in Western Blot (WB). GTX12134

GTX12134 WB Image

WB analysis of (A) Daudi and (B) NIH 3T3 cell lysate using GTX12134 IKB alpha antibody [6A920].
Dilution : 2 μg/ml

Anti-IKB alpha antibody [6A920] used in Western Blot (WB). GTX12134

GTX12134 WB Image

WB analysis of NIH-3T3 cell lysate using GTX12134 IKB alpha antibody [6A920].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399