IKB epsilon (phospho Ser22) antibody

Cat. No. GTX79041

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human
Package	100 μg ($399)

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Datasheet

View product citations for antibody GTX79041 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:1000
IHC-P	1:50 - 1:100

Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human IkB-ε around the phosphorylation site of serine 22 ( I-E-Sp-L-R).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11164236

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

IKBE,NFKB inhibitor epsilon,NFKBIE,IKB epsilon

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011]

Database

Gene ID: 4794 NFKBIE
UniProt: O00221 NFKBIE

Research Area

DATA IMAGES

GTX79041 WB Image

WB analysis of 293 cells untreated or treated with TNF-α (20ng/ml, 15min) lysates using GTX79041 IKB epsilon (phospho Ser22) antibody (Lane 3 and 4) and IKB epsilon antibody (Lane 1 and 2).

GTX79041 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX79041 IKB epsilon (phospho Ser22) antibody.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 399

Qty