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IL1 beta antibody

Orthogonal
Anti-IL1 beta antibody used in Western Blot (WB). GTX22105
Anti-IL1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX22105
Anti-IL1 beta antibody used in Western Blot (WB). GTX22105
Anti-IL1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX22105
Anti-IL1 beta antibody used in Western Blot (WB). GTX22105

Cat. No. GTX22105

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, FACS, IP, ELISA, Neutralizing/Inhibition

Reactivity

 Human, Dog
Package
100 μg ($399)
See all IL1 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:1:100-1:200
IHC-Fr 1:1:100-1:200
FACS Assay dependent
IP 1:400-1:800
ELISA 1:500-1:2000
Neutralizing/Inhibition 1:100
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Product Note

Primarily directed against mature 17kDa human IL-1beta. In ELISA formats and other immunoreactive assays, the antibody will recognize 10% of the non-denatured (native) 31kDa IL-1beta precursor containing samples, but will detect all of the 17kDa mature molecule. Also detects primate IL-1beta in the same formats using similar dilutions.The antiserum does not recognize human IL-1alpha.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was prepared by repeated immunizations with recombinant human IL-1s produced in E.coli. The MW of the recombinant 153 aa IL-1s was 17 kDa with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1s.

Purification

Purified by DEAE chromatography.
From serum

Conjugation

Unconjugated

RRID

AB_368268

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 1 beta , IL-1 , IL1-BETA , IL1F2

Cellular Localization

Cytoplasm, cytosol,Lysosome,Secreted

Background

Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types, and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin1 alpha (IL1 alpha) and interleukin1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25% homology in their amino acid sequence.

Database

Research Area

DATA IMAGES

Anti-IL1 beta antibody used in Western Blot (WB). GTX22105

GTX22105 WB Image

GTX22105 was used at a 1:200 dilution incubated 1 h at room temperature to detect dog IL-1beta by Western blot. Lane 1 and 2 were loaded with 2.5 μg and 500 ng of dog IL-1beta respectively.

Anti-IL1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX22105

GTX22105 IHC-P Image

IHC-P analysis of medullary lymph node using IL1 beta antibody. Positive staining in the cytoplasm of circulating macrophages. Negative Control (far right) normal rabbit IgG.

Anti-IL1 beta antibody used in Western Blot (WB). GTX22105

GTX22105 WB Image

WB analysis of recombinant human IL1 beta protein using GTX22105 IL1 beta antibody.
Loading : 5 ng
Dilution : 1:2000

Anti-IL1 beta antibody used in IHC (Paraffin sections) (IHC-P). GTX22105

GTX22105 IHC-P Image

IHC-P analysis of human medullary lymph node tissue using GTX22105 IL1 beta antibody.

Anti-IL1 beta antibody used in Western Blot (WB). GTX22105

GTX22105 WB Image

Orthogonal

The data was published in the journal Int J Mol Sci in 2019. PMID: 30987270

REFERENCE

Application Reference

Sgrignoli MR et al. Stem Cell Res 2019; 39 : 101525 Reduction in the inflammatory markers CD4, IL-1, IL-6 and TNF帢 in dogs with keratoconjunctivitis sicca treated topically with mesenchymal stem cells.
Application : IHC-P
Reactivity : Dog
Lee YC et al. Int J Mol Sci 2019; 20 (7) Essential Role of Visfatin in Lipopolysaccharide and Colon Ascendens Stent Peritonitis-Induced Acute Lung Injury.
Application : WB
Reactivity : Human
Chu Y et al. Int J Mol Sci 2019; 20 (1) Protective Effects and Mechanisms of N-Phenethyl Caffeamide from UVA-Induced Skin Damage in Human Epidermal Keratinocytes through Nrf2/HO-1 Regulation.
Application : WB
Reactivity : Human
Lai WY et al. Int J Oral Sci 2014; 6 (2) : 34547 An evaluation of the inflammatory response of lipopolysaccharide-treated primary dental pulp cells with regard to calcium silicate-based cements.
Application : WB
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Package List Price ($)
$ 399