GeneTex
United States (US)

IL1 beta recombinant protein

Cat No. GTX65491

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Rat
Species Rat
APPLICATION

Application Note

The ED50 as determined by the dose-dependent stimulation of murine D10S cells is <0.1 ng/ml, corresponding to a specific activity of >1x10^7 units/mg.

Calculated MW

17.3 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Buffer

Sterile filtered and then lyophilized from 10 mM Sodium Acetate, pH 6.5

Storage

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at +4ºC for 1 week or –20ºC for future use.

Antigen Species

Rat

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lymphocyteactivating factor (LAF), Leukocyte Endogenous Mediator (LEM), Catabolin, Endogenous Pyrogen (EP), Mononuclear Cell Factor (MCF), IL1B, Lymphocyte-activating factor (LAF)

Background

IL-1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, B-cell maturation and proliferation, mitogenic FGF-like activity and stimulation of the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. Recombinant rat IL-1beta is a 17.4 kDa protein containing 153 amino acid residues.

Research Area