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IL10 antibody

Anti-IL10 antibody used in Western Blot (WB). GTX130513
Anti-IL10 antibody used in ELISA (ELISA). GTX130513
Anti-IL10 antibody used in IHC (Frozen sections) (IHC-Fr). GTX130513
Anti-IL10 antibody used in IHC (Paraffin sections) (IHC-P). GTX130513

Cat. No. GTX130513

Reference 4
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all IL10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
ELISA 1:-1:10000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Sheep, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human IL10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886295

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 10 , CSIF , GVHDS , IL-10 , IL10A , TGIF

Cellular Localization

Secreted

Background

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IL10 antibody used in Western Blot (WB). GTX130513

GTX130513 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL10 antibody (GTX130513) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IL10 antibody used in ELISA (ELISA). GTX130513

GTX130513 ELISA Image

An ELISA plate is coated with 50 μL of a IL10 peptide at concentration ranged from 0.25 μg/mL to 8 μg/mL. The coated peptide is detected with anti IL10 antibody (GTX130513) at 0.5 μg/mL.

Anti-IL10 antibody used in IHC (Frozen sections) (IHC-Fr). GTX130513

GTX130513 IHC-Fr Image

IL10 antibody detects IL10 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: IL10 stained by IL10 antibody (GTX130513) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-IL10 antibody used in IHC (Paraffin sections) (IHC-P). GTX130513

GTX130513 IHC-P Image

IL10 antibody detects IL10 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
IL10 stained by IL10 antibody (GTX130513) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

De Santa F et al. Microbiome 2024; 12 (1) : 29 Effect of a multi-strain probiotic mixture consumption on anxiety and depression symptoms induced in adult mice by postnatal maternal separation.
Petrella C et al. Pharmacol Res 2021; 172 : 105795 Proneurogenic and neuroprotective effect of a multi strain probiotic mixture in a mouse model of acute inflammation: Involvement of the gut-brain axis.
Application : WB
Reactivity : Mouse
Zhou Y et al. Oncol Lett 2020; 20 (6) : 298 Anti-CD206 antibody-conjugated Fe3O4-based PLGA nanoparticles selectively promote tumor-associated macrophages to polarize to the pro-inflammatory subtype.
Application : WB
Reactivity : Mouse
Xiaopeng Tang et al. bioRxiv 2020; (Epub) Transferrin-dependent crosstalk between the intestinal tract and commensal microbes contributes for immune tolerance.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

IL10 antibody Cat. No. GTX130513

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-IL10 antibody used in Western Blot (WB). GTX130513
Date : Anonymous submitted on 22-Feb-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : colonic mucosa
Amount used : 25 μg
Blocking : 5% fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169