GeneTex
United States (US)

IL12 recombinant protein

Cat No. GTX65485

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Rat
Species Rat
APPLICATION

Application Note

The ED50 range is 0.01-0.05 ng/ml, as determined by the dose-dependent secretion of IFN-gamma from PHA and IL-12 activated mouse splenocytes that has been co-stimulated with PMA.

Calculated MW

70 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Buffer

1 mg/ml solution with no additives.

Storage

Reconstitute in water to a concentration of 0.1-0.5 mg/ml (Note: Gently pipetting to dissolve). The solution can be diluted into other aqueous buffers. Stored at -20ºC for future use.

Antigen Species

Rat

Expression System

Baculovirus (Sf9 insect cells)

Purification


Purity was assessed by SDS-PAGE (≥95%) and by HPLC (≥95%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

IL-12, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, NKSF, IL12, Edodekin-alpha, Edodekinalpha, IL12a, IL12b, IL 12, Edodekin alpha

Background

The recombinant rat IL-12 produced from Sf9 insect cells is a glycosylated disulfide linked hetromeric polypeptide containing 503 amino acids and having a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa and 40 kDa subunits

Research Area

Package List Price ($)
$ 259