The ED50 range is 0.01-0.05 ng/ml, as determined by the dose-dependent secretion of IFN-gamma from PHA and IL-12 activated mouse splenocytes that has been co-stimulated with PMA.
1 mg/ml solution with no additives.
Reconstitute in water to a concentration of 0.1-0.5 mg/ml (Note: Gently pipetting to dissolve). The solution can be diluted into other aqueous buffers. Stored at -20ºC for future use.
Baculovirus (Sf9 insect cells)
Purity was assessed by SDS-PAGE (≥95%) and by HPLC (≥95%).
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
IL-12, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, NKSF, IL12, Edodekin-alpha, Edodekinalpha, IL12a, IL12b, IL 12, Edodekin alpha
The recombinant rat IL-12 produced from Sf9 insect cells is a glycosylated disulfide linked hetromeric polypeptide containing 503 amino acids and having a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa and 40 kDa subunits