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IL17A antibody

Anti-IL17A antibody used in Western Blot (WB). GTX01094
Anti-IL17A antibody used in IHC (Paraffin sections) (IHC-P). GTX01094
Anti-IL17A antibody used in IHC (Paraffin sections) (IHC-P). GTX01094
Anti-IL17A antibody used in IHC (Paraffin sections) (IHC-P). GTX01094
Anti-IL17A antibody used in IHC (Paraffin sections) (IHC-P). GTX01094

Cat. No. GTX01094

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse
Package
100 μg ($419)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.125-0.5 μg/mL
ICC/IF 5-20 μg/mL
IHC-P 2-5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to 16 amino acids near the center of mature human IL-17. The immunogen is located within amino acids 50-100 of IL-17.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 17A , CTLA8 , IL17 , IL17A

Cellular Localization

Secreted

Background

The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IL17A antibody used in Western Blot (WB). GTX01094

GTX01094 WB Image

WB analysis of IL-17 recombinant protein using GTX01094 IL17A antibody.
Loading : 30 ng of human IL-17 recombinant protein per lane
Dilution : 0.125 μg/mL (Lane 1) ; 0.25 μg/mL (Lane 2) ; 0.5 μg/mL (Lane 3)

Anti-IL17A antibody used in IHC (Paraffin sections) (IHC-P). GTX01094

GTX01094 IHC-P Image

IHC-P analysis of formaldehyde-fixed human tonsil tissue using GTX01094 IL17A antibody.
Antigen retrieval : Heat mediation with a citrate buffer (pH6)
Dilution : 5μg/mL

Anti-IL17A antibody used in IHC (Paraffin sections) (IHC-P). GTX01094

GTX01094 IHC-P Image

ICC/IF analysis of 4% PFA-fixed A-20 cells using GTX01094 IL17A antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 5 μg/mL

Anti-IL17A antibody used in IHC (Paraffin sections) (IHC-P). GTX01094

GTX01094 IHC-P Image

IHC-P analysis of 4% PFA-fixed mouse thymus tissue using GTX01094 IL17A antibody.
Dilution : 20μg/mL

Anti-IL17A antibody used in IHC (Paraffin sections) (IHC-P). GTX01094

GTX01094 IHC-P Image

IHC-P analysis of formaldehyde-fixed mouse colon tissue using GTX01094 IL17A antibody.
Antigen retrieval : Heat mediation with a citrate buffer (pH6)
Dilution : 2μg/mL

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419