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IL18 antibody

Anti-IL18 antibody used in Flow cytometry (FACS). GTX03734
Anti-IL18 antibody used in IHC (Paraffin sections) (IHC-P). GTX03734
Anti-IL18 antibody used in Western Blot (WB). GTX03734

Cat. No. GTX03734

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Mouse, Rat
Package
100 μg ($429)
See all IL18 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25-0.5μg/ml
IHC-P 2-5μg/ml
FACS 1-3μg/1x106 cells
ELISA 0.1-0.5μg/ml

Note :

IHC-P
Antigen retireval by heat
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4

Preservative

0.05mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

E.coli-derived rat IL-18 recombinant protein (Position: H37-S194).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 18 , IGIF , IL-18 , IL-1g , IL1F4

Cellular Localization

Secreted

Background

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-IL18 antibody used in Flow cytometry (FACS). GTX03734

GTX03734 FACS Image

FACS analysis of RH35 cells using GTX03734 IL18 antibody.
Antibody amount : 1 μg/1x10⁶ cells
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled sample

Anti-IL18 antibody used in IHC (Paraffin sections) (IHC-P). GTX03734

GTX03734 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX03734 IL18 antibody.
Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution)
Dilution : 2μg/ml

Anti-IL18 antibody used in Western Blot (WB). GTX03734

GTX03734 WB Image

WB analysis of various lysates using GTX03734 IL18 antibody.
Lane 1 : Mouse spleen
Lane 2 : Mouse thymus
Lane 3 : Mouse lung
Lane 4 : ANA-1 cells
Dilution : 0.5 μg/ml
Loading : 30 μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 429