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IL29 antibody

Anti-IL29 antibody used in Western Blot (WB). GTX48682
Anti-IL29 antibody used in Western Blot (WB). GTX48682

Cat. No. GTX48682

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human
Package
50 μg ($399)
See all IL29 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ELISA 1:10000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Mature length recombinant human IL-29 protein produced in E.coli.

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon lambda 1 , IL-29 , IL29

Cellular Localization

Secreted

Background

IL-29 (Interleukin-29) is a secreted cytokine with immunomodulatory activity. It may play a role in antiviral immunity. It up-regulates MHC class I antigen expression. IL-29 is a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Its induction is by viral infections or double stranded RNA.

Database

Research Area

DATA IMAGES

Anti-IL29 antibody used in Western Blot (WB). GTX48682

GTX48682 WB Image

Anti-human IL-29 antibody in western blot shows detection of recombinant human IL-29 raised in E.coli. Recombinant protein (0.1 μg, 19.9 kDa) was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with GeneTex's Anti-Human IL-29 antibody. After washing, membrane was probed with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer) for 30 min. at RT. Data was collected using Bio-Rad VersaDocR 4000 MP imaging system.

Anti-IL29 antibody used in Western Blot (WB). GTX48682

GTX48682 WB Image

WB analysis of recombinant human IL29 protein using GTX48682 IL29 antibody.
Loading : 100 ng

REFERENCE

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Package List Price ($)
$ 399