IL3 Receptor alpha antibody [10B8E7]

Cat. No. GTX60797

Host	Mouse
Clonality	Monoclonal
Clone Name	10B8E7
Isotype	IgG1
Application	WB, ICC/IF, FACS, ELISA
Reactivity	Human
Package	100 μl ($409)

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Datasheet
Document

View product citations for antibody GTX60797 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human IL3RA (AA: 200-305) expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 3 receptor subunit alpha , CD123 , IL3R , IL3RAY , IL3RX , IL3RY , hIL-3Ra

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jun 2012]

Database

Gene ID: 3563 IL3RA
UniProt: P26951 IL3RA

Research Area

DATA IMAGES

GTX60797 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60797 IL3 Receptor alpha antibody [10B8E7].
Green : IL3 Receptor alpha
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

GTX60797 FACS Image

FACS analysis of HeLa cells using GTX60797 IL3 Receptor alpha antibody [10B8E7].
Green : IL3 Receptor alpha
Red : negative control

GTX60797 WB Image

WB analysis of HEK293 (1) and IL3RA (AA: 200-305)-hIgGFc transfected HEK293 (2) cell lysate using GTX60797 IL3 Receptor alpha antibody [10B8E7].

GTX60797 ELISA Image

ELISA analysis of antigen using GTX60797 IL3 Receptor alpha antibody [10B8E7].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 409

Qty