APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified recombinant fragment of human IL3RA (AA: 200-305) expressed in E. Coli.
Purification
Protein G Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin 3 receptor subunit alpha , CD123 , IL3R , IL3RAY , IL3RX , IL3RY , hIL-3Ra
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jun 2012]
Database
Research Area
DATA IMAGES
![Anti-IL3 Receptor alpha antibody [8E11C5] used in Western Blot (WB). GTX60786](/templates/default/js/lightbox/images/loading.gif "Anti-IL3 Receptor alpha antibody [8E11C5] used in Western Blot (WB). GTX60786")
|
GTX60786 WB Image
WB analysis of HEK293 (1) and IL3RA (AA: 200-305)-hIgGFc transfected HEK293 (2) cell lysate using GTX60786 IL3 Receptor alpha antibody [8E11C5].
|
|
|
GTX60786 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX60786 IL3 Receptor alpha antibody [8E11C5].
Green : IL3 Receptor alpha
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
|
|
|
GTX60786 FACS Image
FACS analysis of HeLa cells using GTX60786 IL3 Receptor alpha antibody [8E11C5].
Green : IL3 Receptor alpha
Red : negative control
|
|
|
GTX60786 IHC-P Image
IHC-P analysis of ovarian cancer tissue using GTX60786 IL3 Receptor alpha antibody [8E11C5].
|
|
|
GTX60786 ELISA Image
ELISA analysis of antigen using GTX60786 IL3 Receptor alpha antibody [8E11C5].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
|
|
|
GTX60786 IHC-P Image
IHC-P analysis of lung cancer tissue using GTX60786 IL3 Receptor alpha antibody [8E11C5].
|
REFERENCE
There are currently no references for IL3 Receptor alpha antibody [8E11C5] (GTX60786). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IL3 Receptor alpha antibody [8E11C5] (GTX60786). Be the first to share your experience with this product.
![Anti-IL3 Receptor alpha antibody [8E11C5] used in Western Blot (WB). GTX60786](/upload/website/prouct_img/small/GTX60786/GTX60786_20170912_WB_w_23061123_745.webp "Anti-IL3 Receptor alpha antibody [8E11C5] used in Western Blot (WB). GTX60786")
![Anti-IL3 Receptor alpha antibody [8E11C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60786](/upload/website/prouct_img/small/GTX60786/GTX60786_20170912_ICCIF_w_23061123_531.webp "Anti-IL3 Receptor alpha antibody [8E11C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60786")
![Anti-IL3 Receptor alpha antibody [8E11C5] used in Flow cytometry (FACS). GTX60786](/upload/website/prouct_img/small/GTX60786/GTX60786_20170912_FACS_w_23061123_582.webp "Anti-IL3 Receptor alpha antibody [8E11C5] used in Flow cytometry (FACS). GTX60786")
![Anti-IL3 Receptor alpha antibody [8E11C5] used in IHC (Paraffin sections) (IHC-P). GTX60786](/upload/website/prouct_img/small/GTX60786/GTX60786_20170912_IHC-P_1_w_23061123_787.webp "Anti-IL3 Receptor alpha antibody [8E11C5] used in IHC (Paraffin sections) (IHC-P). GTX60786")
![Anti-IL3 Receptor alpha antibody [8E11C5] used in ELISA (ELISA). GTX60786](/upload/website/prouct_img/small/GTX60786/GTX60786_20170912_ELISA_w_23061123_531.webp "Anti-IL3 Receptor alpha antibody [8E11C5] used in ELISA (ELISA). GTX60786")
![Anti-IL3 Receptor alpha antibody [8E11C5] used in IHC (Paraffin sections) (IHC-P). GTX60786](/upload/website/prouct_img/small/GTX60786/GTX60786_20170912_IHC-P_w_23061123_612.webp "Anti-IL3 Receptor alpha antibody [8E11C5] used in IHC (Paraffin sections) (IHC-P). GTX60786")
![Anti-IL3 Receptor alpha antibody [8E11C5] used in Western Blot (WB). GTX60786](/upload/website/prouct_img/normal/GTX60786/GTX60786_20170912_WB_w_23061123_745.webp "Anti-IL3 Receptor alpha antibody [8E11C5] used in Western Blot (WB). GTX60786")
![Anti-IL3 Receptor alpha antibody [8E11C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60786](/upload/website/prouct_img/normal/GTX60786/GTX60786_20170912_ICCIF_w_23061123_531.webp "Anti-IL3 Receptor alpha antibody [8E11C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60786")
![Anti-IL3 Receptor alpha antibody [8E11C5] used in Flow cytometry (FACS). GTX60786](/upload/website/prouct_img/normal/GTX60786/GTX60786_20170912_FACS_w_23061123_582.webp "Anti-IL3 Receptor alpha antibody [8E11C5] used in Flow cytometry (FACS). GTX60786")
![Anti-IL3 Receptor alpha antibody [8E11C5] used in IHC (Paraffin sections) (IHC-P). GTX60786](/upload/website/prouct_img/normal/GTX60786/GTX60786_20170912_IHC-P_1_w_23061123_787.webp "Anti-IL3 Receptor alpha antibody [8E11C5] used in IHC (Paraffin sections) (IHC-P). GTX60786")
![Anti-IL3 Receptor alpha antibody [8E11C5] used in ELISA (ELISA). GTX60786](/upload/website/prouct_img/normal/GTX60786/GTX60786_20170912_ELISA_w_23061123_531.webp "Anti-IL3 Receptor alpha antibody [8E11C5] used in ELISA (ELISA). GTX60786")
![Anti-IL3 Receptor alpha antibody [8E11C5] used in IHC (Paraffin sections) (IHC-P). GTX60786](/upload/website/prouct_img/normal/GTX60786/GTX60786_20170912_IHC-P_w_23061123_612.webp "Anti-IL3 Receptor alpha antibody [8E11C5] used in IHC (Paraffin sections) (IHC-P). GTX60786")