APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.25-0.5 μg/ml |
| Assay dependent |
| Assay dependent |
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Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.
ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
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Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
200 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human IL3RA protein (around aa 26-171)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
interleukin 3 receptor subunit alpha , CD123 , IL3R , IL3RAY , IL3RX , IL3RY , hIL-3Ra
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jun 2012]
Database
Research Area
DATA IMAGES
![Anti-IL3 Receptor alpha antibody [IL3RA/2947R] used in IHC (Paraffin sections) (IHC-P). GTX02651](/templates/default/js/lightbox/images/loading.gif "Anti-IL3 Receptor alpha antibody [IL3RA/2947R] used in IHC (Paraffin sections) (IHC-P). GTX02651")
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GTX02651 IHC-P Image
IHC-P analysis of human tonsil tissue section using GTX02651 IL3 Receptor alpha antibody [IL3RA/2947R].
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GTX02651 Protein Array Image
Analysis of Protein Array containing more than 19,000 full-length human proteins using IL3RA / CD123 Rabbit Recombinant Monoclonal Antibody (IL3RA /2947R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD') above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD') between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
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REFERENCE
There are currently no references for IL3 Receptor alpha antibody [IL3RA/2947R] (GTX02651). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IL3 Receptor alpha antibody [IL3RA/2947R] (GTX02651). Be the first to share your experience with this product.
![Anti-IL3 Receptor alpha antibody [IL3RA/2947R] used in IHC (Paraffin sections) (IHC-P). GTX02651](/upload/website/prouct_img/small/GTX02651/GTX02651_20210319_IHC-P_w_23053122_664.webp "Anti-IL3 Receptor alpha antibody [IL3RA/2947R] used in IHC (Paraffin sections) (IHC-P). GTX02651")
![Anti-IL3 Receptor alpha antibody [IL3RA/2947R] used in Protein Array (Protein Array). GTX02651](/upload/website/prouct_img/small/GTX02651/GTX02651_20210319_ProteinArray_w_23053122_795.webp "Anti-IL3 Receptor alpha antibody [IL3RA/2947R] used in Protein Array (Protein Array). GTX02651")
![Anti-IL3 Receptor alpha antibody [IL3RA/2947R] used in IHC (Paraffin sections) (IHC-P). GTX02651](/upload/website/prouct_img/normal/GTX02651/GTX02651_20210319_IHC-P_w_23053122_664.webp "Anti-IL3 Receptor alpha antibody [IL3RA/2947R] used in IHC (Paraffin sections) (IHC-P). GTX02651")
![Anti-IL3 Receptor alpha antibody [IL3RA/2947R] used in Protein Array (Protein Array). GTX02651](/upload/website/prouct_img/normal/GTX02651/GTX02651_20210319_ProteinArray_w_23053122_795.webp "Anti-IL3 Receptor alpha antibody [IL3RA/2947R] used in Protein Array (Protein Array). GTX02651")