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IL33 antibody [4E9]

Anti-IL33 antibody [4E9] used in Western Blot (WB). GTX50047

Cat. No. GTX50047

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4E9

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all IL33 products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:500 ~ 1,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human IL-33 (112-270aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 33 , C9orf26 , DVS27 , IL1F11 , NF-HEV , NFEHEV

Cellular Localization

Nucleus , Secreted

Background

The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-IL33 antibody [4E9] used in Western Blot (WB). GTX50047

GTX50047 WB Image

The Cell lysates of Jurkat (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IL-33 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399