APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
|
Note :
Sandwich ELISA
Capture: GTX02997, Detection: GTX02995-02 / GTX02996-02.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody is able to detect native and recombinant human IL-5.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human IL-5
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Biotin
Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
EDF,IL5,TRF,interleukin 5
Cellular Localization
Secreted
Background
This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]
Database
Research Area
DATA IMAGES
![Anti-IL5 antibody [5A10] (Biotin) used in ELISA (ELISA). GTX02995-02](/templates/default/js/lightbox/images/loading.gif "Anti-IL5 antibody [5A10] (Biotin) used in ELISA (ELISA). GTX02995-02")
|
GTX02995-02 ELISA Image
Sandwich ELISA analysis of human IL-5 protein using GTX02997 IL5 antibody [TRFK5] as coating antibody and GTX02995-02 IL5 antibody [5A10] (Biotin) as detecting antibody.
Substrate : pNPP
|
REFERENCE
There are currently no references for IL5 antibody [5A10] (Biotin) (GTX02995-02). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IL5 antibody [5A10] (Biotin) (GTX02995-02). Be the first to share your experience with this product.
![Anti-IL5 antibody [5A10] (Biotin) used in ELISA (ELISA). GTX02995-02](/upload/website/prouct_img/small/GTX02995-02/GTX02995-02_20210507_ELISA_w_23053123_489.webp "Anti-IL5 antibody [5A10] (Biotin) used in ELISA (ELISA). GTX02995-02")
![Anti-IL5 antibody [5A10] (Biotin) used in ELISA (ELISA). GTX02995-02](/upload/website/prouct_img/normal/GTX02995-02/GTX02995-02_20210507_ELISA_w_23053123_489.webp "Anti-IL5 antibody [5A10] (Biotin) used in ELISA (ELISA). GTX02995-02")