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IL7 antibody

Anti-IL7 antibody used in IHC (Paraffin sections) (IHC-P). GTX55679
Anti-IL7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55679
Anti-IL7 antibody used in IHC (Paraffin sections) (IHC-P). GTX55679
Anti-IL7 antibody used in Western Blot (WB). GTX55679

Cat. No. GTX55679

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all IL7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 26-177 of human IL7 (NP_000871.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interleukin 7 , IL-7

Cellular Localization

Secreted

Background

The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.[provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES

Anti-IL7 antibody used in IHC (Paraffin sections) (IHC-P). GTX55679

GTX55679 IHC-P Image

IHC-P analysis of rat liver tissue using GTX55679 IL7 antibody.
Dilution : 1:100

Anti-IL7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55679

GTX55679 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX55679 IL7 antibody.
Blue : DAPI

Anti-IL7 antibody used in IHC (Paraffin sections) (IHC-P). GTX55679

GTX55679 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX55679 IL7 antibody.
Dilution : 1:100

Anti-IL7 antibody used in Western Blot (WB). GTX55679

GTX55679 WB Image

WB analysis of THP-1 cell lysate using GTX55679 IL7 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399