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INF2 antibody

Anti-INF2 antibody used in Western Blot (WB). GTX130714
Anti-INF2 antibody used in Immunoprecipitation (IP). GTX130714

Cat. No. GTX130714

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all INF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

136 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human INF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886328

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inverted formin, FH2 and WH2 domain containing , C14orf151 , C14orf173 , CMTDIE , FSGS5 , pp9484

Background

This gene represents a member of the formin family of proteins. It is considered a diaphanous formin due to the presence of a diaphanous inhibitory domain located at the N-terminus of the encoded protein. Studies of a similar mouse protein indicate that the protein encoded by this locus may function in polymerization and depolymerization of actin filaments. Mutations at this locus have been associated with focal segmental glomerulosclerosis 5.[provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-INF2 antibody used in Western Blot (WB). GTX130714

GTX130714 WB Image

INF2 antibody detects INF2 protein by western blot analysis. Human tissue extracts (30 μg) was separated by 5 % SDS-PAGE, and the membrane was blotted with INF2 antibody (GTX130714) at a dilution of 1:1000.

Anti-INF2 antibody used in Immunoprecipitation (IP). GTX130714

GTX130714 IP Image

Immunoprecipitation of INF2 protein from 293T whole cell extracts using 5 μg of INF2 antibody (GTX130714).
Western blot analysis was performed using INF2 antibody (GTX130714) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169