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ING1 antibody

Anti-ING1 antibody used in Western Blot (WB). GTX23711
Anti-ING1 antibody used in (). GTX23711

Cat. No. GTX23711

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all ING1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ELISA 1:4000-1:14000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Product Note

Reactivity occurs against human p33 ING1 protein and is 100% homologous to all splice variants for ING1.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to an internal region near aa 275-300 of Human p33 ING1 protein (Inhibitor of growth family, member 1).

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

inhibitor of growth family member 1 , p24ING1c , p33 , p33ING1 , p33ING1b , p47 , p47ING1a

Cellular Localization

Nucleus

Background

ING1 is a tumor suppressor/growth regulator that also is reported to play a role in histone acetylation. It cooperates with p53/TP53 in the negative regulatory pathway of cell growth by modulating p53-dependent transcriptional activation. Implicated as a tumor suppressor gene.

Database

Research Area

DATA IMAGES

Anti-ING1 antibody used in Western Blot (WB). GTX23711

GTX23711 WB Image

Western blot analysis is shown using GeneTex's Affinity Purified anti-p33 ING1 antibody to detect over expressed Human ING1 present in cell nuclear extracts. This western blot shows reactivity with purified recombinant GST tagged human ING1b (lane 1) and ING1c protein (lane 2). No reactivity is seen against GST or other ING proteins (not shown). Reactivity of this polyclonal antibody is similar to the specificity of a positive control monoclonal antibody (not shown). The blot was incubated with a 1:1,000 dilution of the antibody at room temperature followed by detection using standard techniques.

Anti-ING1 antibody used in (). GTX23711

GTX23711 Image

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399