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IRE1 alpha antibody

KO/KD
Anti-IRE1 alpha antibody used in Western Blot (WB). GTX30005
Anti-IRE1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX30005
Anti-IRE1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30005
Anti-IRE1 alpha antibody used in Western Blot (WB). GTX30005
Anti-IRE1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX30005
Anti-IRE1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30005
Anti-IRE1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30005
Anti-IRE1 alpha antibody used in Western Blot (WB). GTX30005
Anti-IRE1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30005

Cat. No. GTX30005

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($419)
See all IRE1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-2 μg/ml
ICC/IF 1-2 μg/ml
IHC-P 5 μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A 16 amino acid peptide near the carboxy terminus of human IRE1 alpha.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887585

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

endoplasmic reticulum to nucleus signaling 1 , IRE1 , IRE1P , IRE1a , hIRE1p

Cellular Localization

Endoplasmic reticulum membrane

Background

The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-IRE1 alpha antibody used in Western Blot (WB). GTX30005

GTX30005 WB Image

WB analysis of A20 cell lysate using GTX30005 IRE1 alpha antibody.
Lane A : 0.5 μg/ml
Lane B : 1μg/ml
Lane C : 2μg/ml

Anti-IRE1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX30005

GTX30005 IHC-P Image

IHC-P analysis of human small intestine tissue using GTX30005 IRE1 alpha antibody.
loading: 2 μg/ml

Anti-IRE1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30005

GTX30005 ICC/IF Image

ICC/IF analysis of human small intestine using GTX30005 IRE1 alpha antibody.
loading: 20 μg/ml.
Green: IRE1 alpha Antibody (GTX30005)
Blue: DAPI staining

Anti-IRE1 alpha antibody used in Western Blot (WB). GTX30005

GTX30005 WB Image

KO/KD

WB analysis of HeLa cell or IRE1 alpha KO cell lysates using GTX30005 IRE1 alpha antibody.
Dilution : 0.5μg/mL
Loading : 10μg

Anti-IRE1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX30005

GTX30005 IHC-P Image

IHC-P analysis of rat small intestine tissue using GTX30005 IRE1 alpha antibody.
loading: 2 μg/ml

Anti-IRE1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30005

GTX30005 ICC/IF Image

ICC/IF analysis of A20 cells using GTX30005 IRE1 alpha antibody.
loading: 1 μg/mL.

Anti-IRE1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30005

GTX30005 ICC/IF Image

ICC/IF analysis of rat small intestine tissue using GTX30005 IRE1 alpha antibody.
loading: 20 μg/ml.
Green: IRE1 alpha Antibody (GTX30005)
Blue: DAPI staining

Anti-IRE1 alpha antibody used in Western Blot (WB). GTX30005

GTX30005 WB Image

WB analysis of rat brain tissue lysate using GTX30005 IRE1 alpha antibody.
Lane A : 0.5 μg/ml
Lane B : 1μg/ml

Anti-IRE1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30005

GTX30005 ICC/IF Image

ICC/IF analysis of A20 Cells using GTX30005 IRE1 alpha antibody.
loading: 2 μg/ml

REFERENCE

Application Reference

Lee EJ et al. Sci Rep 2021; 11 (1) : 16356 ATF6 is required for efficient rhodopsin clearance and retinal homeostasis in the P23H rho retinitis pigmentosa mouse model.
Application : WB
Reactivity : Mouse
Huang CY et al. Nutrients 2020; 12 (6) Attenuation of Lipopolysaccharide-Induced Acute Lung Injury by Hispolon in Mice, Through Regulating the TLR4/PI3K/Akt/mTOR and Keap1/Nrf2/HO-1 Pathways, and Suppressing Oxidative Stress-Mediated ER Stress-Induced Apoptosis and Autophagy.
Application : WB
Reactivity : Mouse
Chen JF et al. J Pharm Biomed Anal 2020; 187 : 113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419