APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| 3-6μg/ml |
|
Note :
IHC-P
Human Kidney shows preferential staining in the cytoplasm of DCT.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88335-PEP).
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-SIHYEGSEYKLSHGS, from the internal region of the protein sequence according to NP_004127.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
iron responsive element binding protein 2 , ACO3 , IRE-BP 2 , IRE-BP2 , IRP2 , IRP2AD
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner. Variants in this gene have been associated with lung cancer and chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
|
GTX88335 IHC-P Image
IHC-P analysis of human kidney using GTX88335 IREB2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml
|
|
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GTX88335 WB Image
WB analysis of human liver lysate with (B) and without (A) blocking with the immunising peptide using GTX88335 IREB2 antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for IREB2 antibody, Internal (GTX88335). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for IREB2 antibody, Internal (GTX88335). Be the first to share your experience with this product.
