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IRF3 antibody [GT1194]

Recombinant antibody KO/KD
Anti-IRF3 antibody [GT1194] used in Western Blot (WB). GTX00956
Anti-IRF3 antibody [GT1194] used in Western Blot (WB). GTX00956
Anti-IRF3 antibody [GT1194] used in Western Blot (WB). GTX00956

Cat. No. GTX00956

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1194

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all IRF3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Observed MW

55 kDa. The observed M.W. is based on the following publication. PMID: 30602605

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human IRF3.

Purification


Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon regulatory factor 3 , IIAE7 , IRF3

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Database

Research Area

DATA IMAGES

Anti-IRF3 antibody [GT1194] used in Western Blot (WB). GTX00956

GTX00956 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IRF3 antibody [GT1194] (GTX00956) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed expression level is based on the publication: PMID: 21347389

Anti-IRF3 antibody [GT1194] used in Western Blot (WB). GTX00956

GTX00956 WB Image

WB analysis of various samples using GTX00956 IRF3 antibody [GT1194].
Dilution : 1:1000
Laoding : 25μg

Anti-IRF3 antibody [GT1194] used in Western Blot (WB). GTX00956

GTX00956 WB Image

KO/KD

WB analysis of wild-type (WT) and IRF3 knockout (KO) HeLa cell extracts using GTX00956 IRF3 antibody [GT1194].
Dilution : 1:1000
Laoding : 25μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399