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IRF5 antibody [10T1]

Anti-IRF5 antibody [10T1] used in IHC (Paraffin sections) (IHC-P). GTX57552
Anti-IRF5 antibody [10T1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57552
Anti-IRF5 antibody [10T1] used in Western Blot (WB). GTX57552
Anti-IRF5 antibody [10T1] used in Flow cytometry (FACS). GTX57552

Cat. No. GTX57552

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10T1

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all IRF5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF Assay dependent
IHC-P Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone 10T1 is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IRF-7 protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon regulatory factor 5 , IRF5 , SLEB10

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-IRF5 antibody [10T1] used in IHC (Paraffin sections) (IHC-P). GTX57552

GTX57552 IHC-P Image

IHC-P analysis of Human normal colon mucosa and colon adenocalcinoma using GTX57552 IRF5 antibody.
Antigen retrieval: 0.1M sodium citrate buffer
Dilution: 1:50

Anti-IRF5 antibody [10T1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57552

GTX57552 ICC/IF Image

ICC/IF analysis of THP-1 cells using GTX57552 IRF5 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-IRF5 antibody [10T1] used in Western Blot (WB). GTX57552

GTX57552 WB Image

WB analysis of various samples using GTX57552 IRF5 antibody.
Samples : Ramos, THP-1, A20 and NIH-3T3 whole cell lysate
Dilution : 1:1000

Anti-IRF5 antibody [10T1] used in Flow cytometry (FACS). GTX57552

GTX57552 FACS Image

FACS analysis of THP-1 cells using GTX57552 IRF5 antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399