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ITK antibody [HL1264]

Recombinant antibody Orthogonal
Anti-ITK antibody [HL1264] used in IHC (Paraffin sections) (IHC-P). GTX636666
Anti-ITK antibody [HL1264] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636666
Anti-ITK antibody [HL1264] used in IHC (Paraffin sections) (IHC-P). GTX636666
Anti-ITK antibody [HL1264] used in Western Blot (WB). GTX636666

Cat. No. GTX636666

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1264

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ITK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ITK. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910035

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

IL2 inducible T cell kinase , EMT , LPFS1 , LYK , PSCTK2

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ITK antibody [HL1264] used in IHC (Paraffin sections) (IHC-P). GTX636666

GTX636666 IHC-P Image

ITK antibody [HL1264] detects ITK protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ITK stained by ITK antibody [HL1264] (GTX636666) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ITK antibody [HL1264] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636666

GTX636666 ICC/IF Image

ITK antibody [HL1264] detects ITK protein by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ITK stained by ITK antibody [HL1264] (GTX636666) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ITK antibody [HL1264] used in IHC (Paraffin sections) (IHC-P). GTX636666

GTX636666 IHC-P Image

ITK antibody [HL1264] detects ITK protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
ITK stained by ITK antibody [HL1264] (GTX636666) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ITK antibody [HL1264] used in Western Blot (WB). GTX636666

GTX636666 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITK antibody [HL1264] (GTX636666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations