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IVD antibody

Anti-IVD antibody used in IHC (Paraffin sections) (IHC-P). GTX114502
Anti-IVD antibody used in Western Blot (WB). GTX114502
Anti-IVD antibody used in Western Blot (WB). GTX114502
Anti-IVD antibody used in Western Blot (WB). GTX114502
Anti-IVD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114502

Cat. No. GTX114502

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all IVD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

Mouse brain , PC-12 , HeLa , Jurkat

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IVD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619570

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

isovaleryl-CoA dehydrogenase , ACAD2

Cellular Localization

Mitochondrion matrix

Background

Isovaleryl-CoA dehydrogenase (IVD) is a mitochondrial matrix enzyme that catalyzes the third step in leucine catabolism. The genetic deficiency of IVD results in an accumulation of isovaleric acid, which is toxic to the central nervous system and leads to isovaleric acidemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IVD antibody used in IHC (Paraffin sections) (IHC-P). GTX114502

GTX114502 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using IVD(GTX114502) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IVD antibody used in Western Blot (WB). GTX114502

GTX114502 WB Image

IVD antibody detects IVD protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10 % SDS-PAGE
IVD antibody (GTX114502) dilution: 1:1000

Anti-IVD antibody used in Western Blot (WB). GTX114502

GTX114502 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: JurKat
10% SDS PAGE
GTX114502 diluted at 1:1000

Anti-IVD antibody used in Western Blot (WB). GTX114502

GTX114502 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX114502 diluted at 1:1000

Anti-IVD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114502

GTX114502 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using IVD(GTX114502) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169