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Iaspp antibody

Anti-Iaspp antibody used in IHC (Paraffin sections) (IHC-P). GTX48696
Anti-Iaspp antibody used in Western Blot (WB). GTX48696
Anti-Iaspp antibody used in Western Blot (WB). GTX48696

Cat. No. GTX48696

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
50 μg ($399)
See all IASPP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
IHC-P 1:1000-1:5000
ELISA 1:40000-1:160000
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Product Note

Expect reactivity against both isoform 1 and isoform 2.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to an internal region near amino acids 775-800 of human Iaspp (isoform 1) protein.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 1 regulatory subunit 13 like , IASPP , NKIP1 , RAI , RAI4

Cellular Localization

Cytoplasm,Nucleus

Background

ASPP proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, the third member, iASPP, functionally inactivates p53. iASPP (also called protein phosphatase 1 regulatory (inhibitor) subunit 13 like protein, Inhibitor of ASPP protein, Protein iASPP, PPP1R13B-like protein and NFkB-interacting protein 1) plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. iASPP blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. This protein

Database

Research Area

DATA IMAGES

Anti-Iaspp antibody used in IHC (Paraffin sections) (IHC-P). GTX48696

GTX48696 IHC-P Image

GeneTex's Affinity Purified anti-iASPP antibody shows strong cytoplasmic and membranous staining of myocytes in human heart tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.

Anti-Iaspp antibody used in Western Blot (WB). GTX48696

GTX48696 WB Image

Western blot using GeneTex's affinity purified anti-iASPP antibody shows detection of a band at ~100 kDa (arrowhead) corresponding to isoform 1 of iASPP in MCF7 whole cell lysates. Preincubation with immunizing peptide blocks specific band staining (data not shown). Approximately 35 μg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed with the primary antibody diluted to 1:1,500 in 5% BLOTTO/PBS overnight at 4ºC. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated goat anti-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers. IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Anti-Iaspp antibody used in Western Blot (WB). GTX48696

GTX48696 WB Image

WB analysis of MCF-7 whole cell lysate using GTX48696 Iaspp antibody.
Loading : 35 μg
Dilution : 1:500

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399