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Importin 13 antibody [C3], C-term

Anti-Importin 13 antibody [C3], C-term used in Immunoprecipitation (IP). GTX107982
Anti-Importin 13 antibody [C3], C-term used in Western Blot (WB). GTX107982
Anti-Importin 13 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107982
Anti-Importin 13 antibody [C3], C-term used in Western Blot (WB). GTX107982

Cat. No. GTX107982

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Importin 13 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

108 kDa. ( Note )

Positive Control

IPO13 transfected 293T , PC-12 , Rat2

Predict Reactivity

Mouse, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Importin 13. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950595

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

importin 13 , IMP13 , KAP13 , LGL2 , RANBP13

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the importin-beta family of nuclear transport proteins. The encoded protein mediates the import of specific cargo proteins from the cytoplasm to the nucleus and is dependent on the Ras-related nuclear protein-GTPase system. The encoded protein is also involved in nuclear export of the eukaryotic translation initiation factor 1A.

Database

Research Area

DATA IMAGES

Anti-Importin 13 antibody [C3], C-term used in Immunoprecipitation (IP). GTX107982

GTX107982 IP Image

IPO13 was immunoprecipitated from 1mg of Hela whole cell lysate using 10 ug of anti-IPO13 antibody (GTX107982).
The precipitated IPO13 was detected by GTX107982 diluted at 1:1000.

Anti-Importin 13 antibody [C3], C-term used in Western Blot (WB). GTX107982

GTX107982 WB Image

Western Blot analysis of IPO13 expression in transfected 293T cell line by IPO13 polyclonal antibody.
A: Non-transfected lysate.
B: IPO13 transfected lysate.
7.5% SDS PAGE
GTX107982 diluted at 1:500

Anti-Importin 13 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107982

GTX107982 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using Importin 13(GTX107982) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Importin 13 antibody [C3], C-term used in Western Blot (WB). GTX107982

GTX107982 WB Image

Importin 13 antibody [C3], C-term detects Importin 13 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
7.5 % SDS-PAGE
Importin 13 antibody [C3], C-term (GTX107982) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169